Presidential Young Investigator Award: Protein Expression in E. coli During Transitional Growth.

总统青年研究员奖：大肠杆菌过渡生长期间的蛋白质表达。

• 批准号: 8657533
• 负责人: Michael Domach
• 金额: $ 26.07万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8657533&HistoricalAwards=false
• 关键词: Presidential; Young; Investigator; Award; Protein

Narravtive: This award supports a Presidential Young Investigator (PYI) to study a variety of Biochemical Engineering problems. Current work focuses on cellular processes, enzymology, and developing analysis and evaluation frameworks for plant design. Work that has been completed includes a demonstration that physiological function (e.g. information transduction) linked pathway properties trade off with respect to structure (activity/regulatory mechanism organization) and operating point (metabolite state vector), and fundamental investigations with single and multiactivity enzyme-membrane reactor systems. Future work includes experimental and theoretical studies of underlying molecular and physical processes that play a role in growth kinetics, regulation, and energetics of cells. Novel bioreactors will be devised to study physical, chemical and biological phenomena for a better understanding of reactor operation. Bioprocess design, such as developing frame works whereby the impact on design of key, manipulable processes (cellular, molecular) can be assessed. Also, protein transport phenomena will be investigated to determine how alteration of conformational state and extent of self association influences mobility into/within porous separation media.

旁白：该奖项支持总统青年研究员(PYI)研究各种生化工程问题。目前的工作重点是细胞过程、酶学，以及为植物设计开发分析和评估框架。已经完成的工作包括关于结构(活性/调节机制组织)和操作点(代谢物状态载体)的生理功能(例如信息转导)关联途径特性的权衡，以及单活性和多活性酶-膜反应器系统的基础研究。未来的工作包括对潜在的分子和物理过程的实验和理论研究，这些过程在细胞的生长动力学、调节和能量学中发挥作用。为了更好地了解反应器的运行，将设计新型生物反应器来研究物理、化学和生物现象。生物过程设计，例如开发框架工作，从而可以评估对关键的、可操作的过程(细胞、分子)设计的影响。此外，还将研究蛋白质传输现象，以确定构象状态和自结合程度的变化如何影响进入/进入多孔分离介质的流动性。