Measuring Metabolic Fluxes: Applications for Elucidating Energetics and Regulations

测量代谢通量：阐明能量学和调节的应用

• 批准号: 9222557
• 负责人: Michael Domach
• 金额: $ 18.61万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-15 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9222557&HistoricalAwards=false
• 关键词: Measuring; Metabolic; Fluxes; Applications; Elucidating

This project plans to map the fluxes, enzyme activities, and metabolic levels for key reactions in the glycolytic pathway and the tricarboxylic acid (TCA) cycle for two common bacteria, E. coli and B. subtilis. Both wild-type and metabolic mutants will be examined in order to obtain regulatory insights and alternative yield scenarios. Four areas are expected to be impacted. First, an improved understanding of how the TCA cycle and glycolysis are coordinated would be obtained. Secondly, the magnitude of the energy spilling reactions would be determined. Thirdly, the results could prove useful to developing cultivation conditions that promote the maximal conversion of carbon to cell mass. Finally, metabolic engineering strategies could be identified that would allow one to alter the regulation of the cell in an optimal manner.

该项目计划绘制两种常见细菌（大肠杆菌和枯草芽孢杆菌）糖酵解途径和三羧酸 (TCA) 循环中关键反应的通量、酶活性和代谢水平。 将检查野生型和代谢突变体，以获得监管见解和替代产量方案。 预计四个领域将受到影响。 首先，可以更好地了解 TCA 循环和糖酵解如何协调。 其次，将确定能量溢出反应的幅度。 第三，这些结果可能有助于开发促进碳最大限度地转化为细胞团的培养条件。 最后，可以确定代谢工程策略，使人们能够以最佳方式改变细胞的调节。