Characterization, Manipulation, and Modeling of Membrane- Bound Yeast Alcohol Dehydrogenase Used for a High-Loading Analytical Application

用于高负载分析应用的膜结合酵母醇脱氢酶的表征、操作和建模

• 批准号: 8513443
• 负责人: Michael Domach
• 金额: $ 4.5万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-03-01 至 1987-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8513443&HistoricalAwards=false
• 关键词: Characterization; Manipulation; Modeling; Membrane; Bound

The objective of this research is to characterize, manipulate and model the immobilized enzymes used for a high loading analytical sensing application. The main goal is to experimentally explore the relationship between phenomological performance (activity, lifetime) and enzyme loading, distribution and lability as observed with direct techniques in a slab geometry, membrane system. Experiments will be performed with membrane-bound yeast alcohol dehydrogenase (YADH) that consist of: (1) characterizing the distribution of labelled YADH within the membrane, (2) examining the intrinsic lability of bound YADH with differential scanning calorimetry, and (3) correlating these direct observations with the activity and lifetime of membrane-bound YADH where substrate flux is either convective or diffusive.

本研究的目的是表征、操纵和建模 用于高负载分析传感的固定化酶 应用程序. 主要目标是通过实验探索 表型性能（活性、寿命）之间的关系 和酶负载，分布和不稳定性，如直接 技术在一个平板几何，膜系统。 实验将 用膜结合酵母醇脱氢酶（YADH）进行， 包括：（1）表征标记的YADH在体内的分布 膜，（2）检查结合YADH的内在不稳定性， 差示扫描量热法，和（3）关联这些直接 膜结合YADH的活性和寿命的观察 其中基底通量是对流的或扩散的。