Genetic Hypervariability in Halobacterium Halobium

盐杆菌的遗传高变性

• 批准号: 8703486
• 负责人: Shiladitya DasSarma
• 金额: $ 23.1万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-15 至 1991-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8703486&HistoricalAwards=false
• 关键词: Genetic; Hypervariability; Halobacterium; Halobium

The extremely halophilic archaebacterium, Halobacterium halobium, has the capability to synthesize intracellular gas-filled vesicles for buoyancy to regulate the availability of light and oxygen. The mutation rate for gas vesicle synthesis (Vac) is the highest of several phenotypic hypervariabilities observed in H. halobium. The Vac mutation rate (1%) is 100-fold higher than for the well-characterized purple membrane mutations which are the result of insertional inactivation of the bacterio-opsin (bop gene (DasSarma, et al., unpublished), we are now in an ideal position to study the mutation, structure, and regulation of the gvh gene using standard methods of molecular genetics. We plan to: (a) examine the physical and transcriptional state of the gvh gene region in many Vac mutants by Southern and Northern hybridizations; and (b) complete the DNA sequence of the gvh gene region and determine the structure of the mRNA by mapping with S1 nuclease and capping enzyme.

极端嗜盐的古细菌，盐生盐生细菌，有能力合成细胞内充满气体的小泡，以调节光和氧气的可获得性。气泡合成(Vac)的突变率是在盐生梭菌中观察到的几个表型超变量中最高的。VAc突变率(1%)是特征良好的紫膜突变的100倍，紫膜突变是细菌视蛋白(bop基因)插入失活的结果(DasSarma等人，未发表)，我们现在处于理想的位置，用标准的分子遗传学方法研究GVH基因的突变、结构和调控。我们计划：(A)通过Southern和Northern杂交检测许多Vac突变体中GVH基因区域的物理和转录状态；以及(B)完成GVH基因区域的DNA序列，并通过S1核酸酶和封闭酶定位来确定mRNA的结构。