Collaborative Research: Sequencing and Functional Genomics of Halobacterium

合作研究：盐杆菌的测序和功能基因组学

• 批准号: 9812330
• 负责人: Shiladitya DasSarma
• 金额: $ 29.68万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9812330&HistoricalAwards=false
• 关键词: Collaborative; Research; Sequencing; Functional; Genomics

The goal of this project is to sequence the genome of Halobacterium, an extremely halophilic Archaeon, in its entirety and initiate functional genomic studies. The genome of Halobacterium NRC-1 contains a stable 2.0 Mbp circular chromosome and two large unstable plasmids, pNRC100 and pNRC200, 191 kb and 300 kb, respectively. The justification for this project is: (a) Halobacterium represents one of the best model Archaeal systems, with well-developed genetic methodologies such as genetic transformation and gene replacement and overexpression; (b) Halobacterium represents a major physiological type (extreme halophile) of the Archaea; (c) Halobacterium has many unique and unusual biochemical and cellular characteristics, including the light-driven proton pump bacteriorhodopsin, other retinal proteins, and salt-loving enzymes, etc.; (d) Halobacterium has a genome that is the smallest among halophiles and has been extensively characterized by mapping and sequencing; (e) Halobacterium has a genuine potential for biotechnology, for example in bioengineering of unusual characteristics for special applications (such as bacteriorhodopsin films for biological computers and programmed cell flotation for bioremediation), and as a host for productionof novel biological products (such as biodegradable plastics and recombinant proteins and enzymes); and (f) Halobacterium exhibits salt tolerance that may be genetically transferable to crop plants. The majority of the DNA sequencing work will be conducted in the Hood lab at the University of Washington while most of the computational analysis of the sequence and functional genomics will be conducted in the DasSarma laboratory. Resources will be shared between the two groups and the DNA sequence will be released to the scientific community, ensuring a dynamic project with significant long-term scientific impact.

该项目的目标是对极端嗜盐的古生代盐生细菌的基因组进行完整测序，并启动功能基因组研究。盐生杆菌NRC-1基因组包含一个稳定的2.0Mbp的环状染色体和两个大的不稳定的质粒pNRC100和pNRC200，分别为191kb和300kb。该项目的理由是：(A)盐杆菌是古生菌系统的最佳模式之一，具有成熟的遗传方法，如遗传转化、基因替换和过度表达；(B)盐杆菌是古生界的一个主要生理类型(极端嗜盐菌)；(C)盐杆菌具有许多独特和不同寻常的生化和细胞特征，包括光驱动的质子泵细菌视紫红质、其他视网膜蛋白和嗜盐酶等；(D)盐杆菌的基因组是嗜盐菌中最小的，并已通过作图和测序进行了广泛的特征描述；(E)盐杆菌在生物技术方面具有真正的潜力，例如，在具有特殊应用特性的生物工程方面(如用于生物计算机的细菌视紫红质薄膜和用于生物修复的程序化细胞浮选)，以及作为生产新型生物制品(如可生物降解塑料和重组蛋白质和酶)的宿主；和(F)盐杆菌表现出可遗传给农作物的耐盐性。大部分DNA测序工作将在华盛顿大学的胡德实验室进行，而对序列和功能基因组学的大部分计算分析将在DasSarma实验室进行。资源将在两个小组之间共享，DNA序列将向科学界发布，确保一个具有重大长期科学影响的充满活力的项目。