Tissue-Specific and Developmental Expression of Lectin Genesin Cereals and Rice

凝集素 Genesin 谷物和水稻的组织特异性和发育表达

• 批准号: 8703513
• 负责人: Natasha Raikhel
• 金额: $ 18万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1990-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8703513&HistoricalAwards=false
• 关键词: Tissue; Specific; Developmental; Expression; Lectin

Tissue-Specific and Developmental Expression of Lectin Genes in Cereals and Rice: Molecular genetics and immuno cytochemical approaches will be used to study processes that control tissue- specific gene expression of lectin synthesis. The approach will consist of the following stages; a) characterization of genomic sequences of wheat germ agglutinin and rice lectins; b) analysis and mutagenesis of promoter sequences of these genes, c) transformation experiments to examine how specific gene constructs are expressed. The differential localization of lectins among different cells of closely related species of crop grasses provides a model system for studying cell-and tissue-specific expression of closely related proteins. It provides a unique opportunity to answer questions important to the genetic engineering of plants e.g. How will wheat germ agglutinin genes be expressed when introduced into rice? Will the pattern be similar to that found in wheat or in rice? What are the gene sequences responsible for determining the pattern of expression of similar proteins in different tissues? Finally the potential creation of lectin- deficient plants could possibly answer the perplexing riddle of lectin function in organisms.

凝集素基因的组织特异性和发育表达 谷物和水稻：分子遗传学和免疫细胞化学 方法将用于研究控制组织的过程- 凝集素合成的特异性基因表达。 该办法将 由以下阶段组成：a）基因组的表征 麦胚凝集素和稻凝集素的序列; B）分析 和这些基因的启动子序列的诱变，c） 转化实验来检测特定基因 构建表达。 凝集素在不同植物中的差异定位 近缘禾本科作物的细胞提供了一个模型 研究细胞和组织特异性表达的系统 密切相关的蛋白质。 它提供了一个独特的机会， 回答植物基因工程的重要问题 例如，小麦胚芽凝集素基因在 引入水稻？ 图案会不会和我们发现的 小麦还是大米 是什么基因序列 确定相似蛋白质的表达模式， 不同的组织 最后，凝集素的潜在产生- 有缺陷的植物可能会回答这个令人困惑的谜题， 凝集素在生物体中的功能。