Targeting to the Vacuoles in Plant Cells

针对植物细胞中的液泡

• 批准号: 9002652
• 负责人: Natasha Raikhel
• 金额: $ 40.29万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-15 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9002652&HistoricalAwards=false
• 关键词: Targeting; Vacuoles; Plant; Cells

Comparison of the amino acid sequences of plant vacuolar proteins indicates that there is no sequence in common among these proteins which can be solely responsible for targeting to vacuoles. Therefore, the study of sorting signals from different proteins will be required to understand the structural determinants involved in this process. Gramineae lectins, which are vacuolar proteins, are among the best characterized plant proteins. Amino acid sequences, 3-D protein structures, and cDNAs for these lectins are available. Transgenic tobacco plants and suspension cultures have been obtained which correctly express and process barley lectin. The goals of this research for the next five years include the analysis and characterization of protein sequences responsible for sorting to the vacuole and the identification of a putative receptor (or receptors) which recognizes these sorting sequences and subsequently mediates protein transport to vacuoles. The vacuolar sorting signal of the barley lectin proprotein will be identified and analyzed. Once the sorting signal is identified, the following questions will be addressed: 1) What is the shortest sequence capable of redirecting a reporter protein to the vacuole? 2) What are the specific amino acids within the sorting domain that are responsible for correct targeting? 3) Is secondary structure of the sorting signal important? The characteristics of binding of probarley lectin to its putative receptor(s) will also be determined. Maintenance of separate subcellular compartments in eukaryotic cells depends on the correct sorting and targeting of newly synthesized proteins. Thus, mechanisms must exist in the cell to assure that these proteins are targeted to the correct intracellular membranes. In plants, many proteins are deposited in multifunctional organelles called vacuoles. These proteins contribute to the lysosomal, storage, and plant defense functions of the vacuole. The goal of this research is to understand the mechanisms by which proteins are targeted to the plant cell vacuole. The results will contribute important new information on this important problem in plant cell biology.

植物液泡蛋白氨基酸序列的比较 表明这些序列之间没有共同的序列 这些蛋白质可以单独负责靶向 空泡 因此，对来自不同信号的分选的研究 蛋白质将被要求了解结构 参与这一过程的决定因素。 禾本科植物凝集素， 是液泡蛋白，是植物中最具特征的蛋白质之一， proteins. 氨基酸序列，三维蛋白质结构， 这些凝集素的cDNA是可获得的。 转基因烟草植物 获得了悬浮培养物， 表达和加工大麦凝集素。 这项研究的目的是 未来五年，包括分析和表征 蛋白质序列负责分类到液泡， 鉴定推定的受体（或多种受体）， 识别这些排序序列并随后介导 蛋白质运输到液泡。 的液泡分选信号 大麦凝集素前蛋白将被鉴定和分析。 一旦识别出分选信号， 将解决：1）什么是最短的序列能够 将报告蛋白重定向到液泡 2)有哪些 分选结构域内的特定氨基酸， 负责正确的定位？ 3)二级结构是 分类信号很重要吗 的装订特点 还将通过将probarley凝集素与其推定受体结合来抑制probarley凝集素的活性。 测定 真核生物中不同亚细胞区室的维持 细胞依赖于正确的分选和靶向新的 合成蛋白质。 因此，细胞中必须存在机制， 确保这些蛋白质靶向正确的目标 细胞内膜 在植物中，许多蛋白质 在多功能细胞器中称为液泡。 这些蛋白质 有助于溶酶体，储存和植物防御功能 vacuole. 这项研究的目的是了解 蛋白质靶向植物细胞的机制 空泡 结果将提供重要的新信息 这个重要的植物细胞生物学问题。