U.S. Czech Plant Research on Subcellular Compartmentation of Phytohormone Conjugation

INT 9600462 Raikhel密歇根州立大学的Natasha Raikhel博士和布尔诺市捷克生物物理研究所的Bretislav Brzobohty博士共同开展的这一美国-捷克研究项目，将研究调节植物发育特征的植物激素。具体地说，研究人员打算研究亚细胞区隔在调节植物激素活性中所起的作用。这将通过可逆连接完成，使用克隆的玉米细胞分裂素葡萄糖苷酶(cDNA，Zm-p60.1)。在这个过程中将确定细胞分裂素释放的程度，并将分析观察到的由释放的细胞分裂素引起的生理反应。初步发现将有助于研究小组构建表达Zm-p60.1在不同亚细胞中积累的转基因植物并对其进行分子表征。这项研究有望加深我们对细胞分裂素在植物从营养阶段到生殖阶段的发育转换中所起作用的基本理解。对释放的细胞分裂素对花朵过渡、根发育、种子生产和光合作用能力等活动的各种影响的新认识可能会导致控制作物中一些重要农业性状的结果。这个植物细胞科学项目通过使美国和东欧的顶尖专家能够结合互补的人才，并在共同感兴趣和能力的领域汇集研究资源，实现了促进科学知识发展的计划目标。?？

INT 9600462 Raikhel This U.S.-Czech research project between Dr. Natasha Raikhel of Michigan State University and Dr. Bretislav Brzobohaty of the Czech Institute of Biophysics, Brno, will examine plant hormones that mediate features of plant development. Specifically, the researchers intend to study the role played by subcellular compartmentation in regulation of phytohormone activity. This will be done by reversible conjugation, using a cloned maize cytokinin glucosidase (cDNA, Zm-p60.1). The extent of the cytokinin release will be determined in the process and the observed physiological responses caused by the released cytokinins will be analyzed. Initial findings will contribute to the research team's efforts to construct and molecularly characterize transgenic plants that express accumulations of Zm-p60.1 in various subcellular compartments. The research is expected to improve our fundamental understanding of the cytokinin role in the developmental switch in plants from the vegetative to the reproductive phase. New knowledge of various effects of released cytokinins on activities such as floral transition, root development, seed production, and photosynthetic capacity may lead to results that permit control of some agriculturally important traits in crops. This project in plant cellular science fulfills the program objective of advancing scientific knowledge by enabling leading experts in the United States and Eastern Europe to combine complementary talents and pool research resources in areas of mutual interests and competence. ??