U.S. Czech Plant Research on Subcellular Compartmentation of Phytohormone Conjugation

INT 9600462 Raikhel 这个美国-密歇根州立大学的Natasha Raikhel博士和捷克布尔诺生物物理研究所的Bretislav Brzobohaty博士之间的捷克研究项目将研究调节植物发育特征的植物激素。 具体来说，研究人员打算研究亚细胞区室化在调节植物激素活性中所起的作用。 这将通过使用克隆的玉米细胞分裂素葡糖苷酶（cDNA，Zm-p60.1）的可逆缀合来完成。 将在该过程中确定细胞分裂素释放的程度，并分析所观察到的由释放的细胞分裂素引起的生理反应。 初步的发现将有助于研究小组努力构建和分子表征在各种亚细胞区室中表达Zm-p60.1积累的转基因植物。 该研究有望提高我们对细胞分裂素在植物从营养生长到生殖生长发育转换中的作用的基本认识。 释放的细胞分裂素对花的转变、根的发育、种子的产生和光合能力等活动的各种影响的新知识可能导致允许控制作物中某些农业上重要的性状的结果。 该植物细胞科学项目通过使美国和东欧的领先专家能够在共同利益和能力的领域联合收割机互补人才和汇集研究资源，实现了推进科学知识的计划目标。 ??

INT 9600462 Raikhel This U.S.-Czech research project between Dr. Natasha Raikhel of Michigan State University and Dr. Bretislav Brzobohaty of the Czech Institute of Biophysics, Brno, will examine plant hormones that mediate features of plant development. Specifically, the researchers intend to study the role played by subcellular compartmentation in regulation of phytohormone activity. This will be done by reversible conjugation, using a cloned maize cytokinin glucosidase (cDNA, Zm-p60.1). The extent of the cytokinin release will be determined in the process and the observed physiological responses caused by the released cytokinins will be analyzed. Initial findings will contribute to the research team's efforts to construct and molecularly characterize transgenic plants that express accumulations of Zm-p60.1 in various subcellular compartments. The research is expected to improve our fundamental understanding of the cytokinin role in the developmental switch in plants from the vegetative to the reproductive phase. New knowledge of various effects of released cytokinins on activities such as floral transition, root development, seed production, and photosynthetic capacity may lead to results that permit control of some agriculturally important traits in crops. This project in plant cellular science fulfills the program objective of advancing scientific knowledge by enabling leading experts in the United States and Eastern Europe to combine complementary talents and pool research resources in areas of mutual interests and competence. ??