Osmotic Regulation in Freshwater and Salt Tolerant Charophyte Algae

淡水和耐盐性轮藻的渗透调节：耐盐性对于决定植物的自然分布和在盐碱地上决定农作物的产量是重要的，盐碱地自然是盐碱地或灌溉产生的。藻类为研究耐盐性提供了一个模型系统，因为可以更精确地施加胁迫，并可以更详细地分析响应。两种密切相关的轮藻在耐盐性上存在差异：珊瑚轮藻是一种淡水藻类，不调节其膨大；巴克利轮藻耐盐，能够调节膨大。通过研究这些物种之间的差异，这些研究将阐明膨胀率调节的机制。研究的目的是通过存活率、膜电位和离子组成进一步表征这两个物种在调节膨压和抵抗高盐浓度方面的能力的差异；利用膜片钳鉴定对膨压调节具有重要作用的运输系统，并分离和鉴定质膜上可能为运输系统提供能量的H+-ATPase。

Osmotic Regulation in Freshwater and Salt Tolerant Charophyte Algae: Salt tolerance is important in determining the natural distribution of plants and in determining yield of agricultural crop plants in saline soils which are naturally saline or arise from irrigation. Algae provide a model system for studying salt tolerance, because stresses can be applied more precisely and responses can be analyzed in more detail. Two closely related species of Charophyte algae differ in their salt tolerance: Chara corallina is a freshwater alga that does not regulate its turgor; C. buckellii is salt tolerant and is able to regulate turgor. By studying the difference between these species these studies will elucidate the mechanism of turgor regulation. The research objectives are to further characterize the difference between the two species with respect to their ability to regulate turgor and withstand high salt concentration, using survivorship, membrane potentials, and ion composition; to identify transport systems important to turgor regulation using patch clamp and to isolate and characterize the H+-ATPase of the plasmalemma which may provide energy for transport systems.