Osmotic Regulation in Freshwater and Salt Tolerant Charophyte Algae

耐淡水和耐盐轮藻的渗透调节

• 批准号: 8703876
• 负责人: Mary Bisson
• 金额: $ 19.9万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8703876&HistoricalAwards=false
• 关键词: Osmotic; Regulation; Freshwater; Salt; Tolerant

Osmotic Regulation in Freshwater and Salt Tolerant Charophyte Algae: Salt tolerance is important in determining the natural distribution of plants and in determining yield of agricultural crop plants in saline soils which are naturally saline or arise from irrigation. Algae provide a model system for studying salt tolerance, because stresses can be applied more precisely and responses can be analyzed in more detail. Two closely related species of Charophyte algae differ in their salt tolerance: Chara corallina is a freshwater alga that does not regulate its turgor; C. buckellii is salt tolerant and is able to regulate turgor. By studying the difference between these species these studies will elucidate the mechanism of turgor regulation. The research objectives are to further characterize the difference between the two species with respect to their ability to regulate turgor and withstand high salt concentration, using survivorship, membrane potentials, and ion composition; to identify transport systems important to turgor regulation using patch clamp and to isolate and characterize the H+-ATPase of the plasmalemma which may provide energy for transport systems.

淡水耐盐植物的渗透调节 轮藻：耐盐性在决定 植物的自然分布和确定产量 农业作物植物在盐渍土，这是自然的 盐水或来自灌溉。 藻类提供了一个模型系统 研究耐盐性，因为可以施加压力， 并且可以更详细地分析响应。 两种密切相关的轮藻藻类在它们的 耐盐性：Chara correra是一种淡水藻类， 不调节其膨压; C. Buckellii是耐盐的， 能够调节膨压。 通过研究 这些研究将阐明这些物种的机制 膨压调节 研究目的是进一步表征 两个物种之间的差异， 调节膨压和耐高盐的能力 浓度，使用存活率，膜电位和离子 组成;以确定重要的运输系统膨压 调节使用膜片钳和分离和表征 质膜的H+-ATP酶可能提供能量， 运输系统。