Negative Ion Mass Spectrometry in Peptide Sequencing

将开发一种新的肽测序方法， 负离子质谱 负化学电离 质谱（NCI-MS）可以提供显着的增强 保持与机械和机械同步所需的灵敏度 自动蛋白质测序的工程改进。 为 分子是强烈的电子捕获，NCI-MS已被 据报道，其检测极限为飞摩尔至阿摩尔 （10-18）范围。 由于电离的选择性 过程中，背景噪声减少，进一步增强信号 噪声比。 拟议的研究将涉及探索一个 异硫氰酸苯酯（PITC） 衍生物通过NCI-MS，并表现出高度灵敏的 用于蛋白质和肽测序的检测方法。 蛋白质测序对于理解 蛋白质结构中的修饰氨基酸，并提供 用于克隆基因的探针以及划定边界 编码蛋白质的DNA片段。 本文书 发展建议还寻求能够与远 比目前的努力更少的材料， 微量化学

A new method for peptide sequencing will be developed involving negative ion mass spectrometry. Negative chemical ionization mass spectrometry (NCI-MS) can provide the dramatic enhancement in sensitivity necessary to keep pace with the mechanical and engineering improvements in automated protein sequencing. For molecules which are strongly electron-capturing, NCI-MS has been reported to have detection limits in the femtomole to attomole (10-18) range. Because of the selectivity of the ionization process, background noise is diminished, further enhancing signal to noise ratio. The proposed research will involve exploring a variety of electron-capturing phenylisothiocyanate (PITC) derivatives by NCI-MS and demonstrate a highly sensitive detection method for use in protein and peptide sequencing. protein sequencing is important for an understanding of the roles of modified amino acids in protein structure and to provide probes for cloning genes as well as to deliminate the boundaries of DNA segments coding for proteins. This instrument development proposal seeks also to be able to work with far smaller amounts of material than current efforts in microchemistry.