Fundamental Studies of Polymeric Biphasic Systems for Bioseparations

用于生物分离的聚合物双相系统的基础研究

• 批准号: 8708839
• 负责人: James Hsu
• 金额: $ 7.5万
• 依托单位: Lehigh University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-06-15 至 1990-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8708839&HistoricalAwards=false
• 关键词: Fundamental; Studies; Polymeric; Biphasic; Systems

The manufacturing of substances of biological origin using genetic engineering methodology depends on efficient separation and purification techniques. This proposal seeks to systematize a purification methodology based on a biomolecular partition between two or more immiscible phases in an aqueous system. The specific objectives of this proposal are to: - Characterize aqueous biphasic systems using polymer solution thermodynamics. - Predict partition coefficients using the properties of proteins at surfaces. The success of these studies will strengthen our understanding of the molecular mechanisms and interactions of aqueous two-phase systems. In addition, it will provide engineering principles for selecting the proper system for effective separations at analytical and commercial scale operations. The PI is considered to be well qualified to carry out the proposed research and I recommend funding of this proposal.

使用基因工程方法制造生物来源的物质依赖于有效的分离和提纯技术。这项提议试图系统化一种基于水体系中两个或更多不相容相之间的生物分子分配的纯化方法。这项建议的具体目标是：-使用聚合物溶液热力学表征双水相体系。-利用蛋白质在表面的性质预测分配系数。这些研究的成功将加深我们对双水相体系的分子机理和相互作用的理解。此外，它还将为在分析和商业规模操作中选择有效分离的适当系统提供工程原则。私人投资委员会被认为完全有资格进行拟议的研究，我建议为这项建议提供资金。