Fundamental Studies of Aqueous Polymeric Two-Phase Systems for Bioseparations

用于生物分离的水性聚合物两相系统的基础研究

• 批准号: 8702912
• 负责人: James Hsu
• 金额: $ 19.97万
• 依托单位: Lehigh University
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-05-01 至 1991-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8702912&HistoricalAwards=false
• 关键词: Fundamental; Studies; Aqueous; Polymeric; Two

The quality of the products of modern biotechnology often are limited by the effectiveness of the separation operations. The effectiveness of the separations technology is central to the biological activity, purity and safety of the products. Since biological materials must be concentrated and separated in their natural environment, special techniques must be developed for their processing and treatment. This program addresses one such need and focuses on a multiphase extraction system consisting of two aqueous (water) phases in which the proteins of interest are handled gently without denaturation. The research will explore how the protein can be segregated into one of the two phases and could be purified by settling under the influence of gravity. Thus no special machinery would be needed in the separation step.

现代生物技术产品的质量往往 受分离操作的有效性的限制。 的 分离技术的有效性是 产品的生物活性、纯度和安全性。 以来 生物材料必须集中和分离， 在自然环境中，必须开发特殊技术， 他们的处理和治疗。 该方案解决了这样一个 需要并关注多相提取系统，该多相提取系统包括 两个水（水）相，其中目的蛋白质 轻轻处理而不变性。 该研究将探索 蛋白质如何被分离成两个相之一， 可以通过在重力的影响下沉降来纯化。 因此，在分离步骤中将不需要特殊的机器。