Genetic and Molecular Analysis of a Yeast Glucose Transporter

酵母葡萄糖转运蛋白的遗传和分子分析

• 批准号: 8709915
• 负责人: Marian Carlson
• 金额: $ 15万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-10-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8709915&HistoricalAwards=false
• 关键词: Genetic; Molecular; Analysis; Yeast; Glucose

These studies of the global regulatory circuitry for the glucose response have led to the identification of a gene (SNF3) from yeast that is essential for high-affinity glucose transport. It has been cloned and the sequence analysis indicates that it encodes a membrane protein with homology to a major human glucose transporter. Thus, the evidence indicates that the SNF3 gene encodes a glucose transport protein. SNF3 is the first glucose transporter gene identified in yeast. The structure, regulation and function of this gene and its protein product will be studied using the powerful genetic and molecular techniques available in yeast. The objective is to understand the role of the SNF3 protein in the response to glucose availability.%%% The analysis of a yeast glucose transporter will lead to an understanding of mechanisms by which eukaryotic cells respond to changes in glucose availability. This laboratory has carried out a genetic and molecular genetic analysis of the glucose regulatory response in yeast (Saccharomyces cerevisiae). This organism was chosen to study because the genetics of this small eukaryote is highly developed. Thus biochemical and molecular methods, and also very powerful genetic approaches can be brought to bear on the problem. Studies in recent years have shown that yeast shares many of the key features of higher eukaryotic cells; thus, much of what is learned will also apply to mammalian cells.***

这些研究的全球调节电路， 葡萄糖反应导致了一个基因的鉴定 （SNF 3），是高亲和力葡萄糖所必需的 运输 该基因已被克隆并进行了序列分析 表明它编码一种膜蛋白， 一种主要的人类葡萄糖转运蛋白 因此，证据 表明SNF 3基因编码葡萄糖转运蛋白 蛋白 SNF 3是第一个葡萄糖转运蛋白基因 在酵母中鉴定。 结构、调节和功能 该基因及其蛋白质产物的研究将使用 强大的基因和分子技术， 酵母 目的是了解SNF 3的作用 蛋白质对葡萄糖可用性的反应。% 对酵母葡萄糖转运蛋白的分析将导致 了解真核细胞的反应机制 葡萄糖利用率的变化。 本实验室曾 进行了遗传和分子遗传分析， 酵母（Saccharomyces cerevisiae）。 选择这种生物进行研究是因为 这种小型真核生物的遗传学非常发达。 因此，生物化学和分子方法，也非常 强大的遗传学方法可以用来影响 问题. 近年来的研究表明，酵母 具有高等真核细胞的许多关键特征; 因此，所学到的许多知识也适用于哺乳动物， 细胞。*