RUI: Photoaffinity Labeling of T Lymphocyte Activation Complex

RUI：T 淋巴细胞激活复合物的光亲和标记

• 批准号: 8710424
• 负责人: R. Blake Whitaker
• 金额: $ 11.56万
• 依托单位: Bates College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-15 至 1990-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8710424&HistoricalAwards=false
• 关键词: RUI; Photoaffinity; Labeling; Lymphocyte; Activation

The cell surface structures that mediate the activation of T lymphocytes have been identified by genetic means. To extend our understanding of the function of this complex of molecules, information on the physiology of interaction between these structures and their ligand, antigen, must be developed. Identification of the antigen binding sites possessed by the genetically identified structures will be accomplished by utilizing an antigen that can covalently bind to such structures when irradiated with visible light. Specifically, sulfo.SANPAH immune T lymphocytes will be incubated with sulfo.SANPAH, irradiated, and the sulfo.SANPAH labelled structures will be identified immunochemically. A similar approach will be employed to examine antigen presentation. The information derived will contribute fundamental knowledge basic to our understanding of the ontogeny and regulation of the immune system. Since this research will be carried out at an undergraduate institution, it will also provide undergraduate students with exposure to research on a problem at the forefront of modern immunobiology.

介导T细胞活化的细胞表面结构 淋巴细胞已经通过遗传方法鉴定。 表示我们 了解这种分子复合体的功能， 关于这些之间相互作用的生理学信息， 结构和它们的配体，抗原，必须开发。 抗原结合位点的鉴定 基因识别结构将通过 利用可以共价结合到这些结构上的抗原 当用可见光照射时。 特别是磺基。SANPAH 将免疫T淋巴细胞与磺基SANPAH一起孵育， 辐照，并将磺基SANPAH标记的结构 免疫化学鉴定。 将采用类似的方法 检测抗原呈递 所获得的信息将有助于基础知识 这是我们了解个体发育和调节的基础。 免疫系统 由于这项研究将在一个 本科院校，它也将提供本科 接触前沿问题研究的学生 现代免疫生物学。