RUI: Photoaffinity Labeling of T Lymphocyte Activation Complex

介导T淋巴细胞活化的细胞表面结构已经通过遗传手段得到了鉴定。为了扩大我们对这种分子复合体的功能的理解，必须发展关于这些结构和它们的配体抗原之间相互作用的生理学信息。通过利用一种在可见光照射下可以共价结合到这些结构上的抗原，将完成对遗传识别结构所具有的抗原结合部位的鉴定。具体地说，硫磺.SANPAH免疫T淋巴细胞将与硫磺.SANPAH、照射后的硫酸盐以及硫酸盐.SANPAH标记的结构进行免疫化学鉴定。类似的方法将被用来检查抗原呈递。所获得的信息将有助于我们了解免疫系统的个体发育和调节的基本知识。由于这项研究将在本科院校进行，它也将为本科生提供接触到现代免疫生物学前沿问题的研究机会。

The cell surface structures that mediate the activation of T lymphocytes have been identified by genetic means. To extend our understanding of the function of this complex of molecules, information on the physiology of interaction between these structures and their ligand, antigen, must be developed. Identification of the antigen binding sites possessed by the genetically identified structures will be accomplished by utilizing an antigen that can covalently bind to such structures when irradiated with visible light. Specifically, sulfo.SANPAH immune T lymphocytes will be incubated with sulfo.SANPAH, irradiated, and the sulfo.SANPAH labelled structures will be identified immunochemically. A similar approach will be employed to examine antigen presentation. The information derived will contribute fundamental knowledge basic to our understanding of the ontogeny and regulation of the immune system. Since this research will be carried out at an undergraduate institution, it will also provide undergraduate students with exposure to research on a problem at the forefront of modern immunobiology.