Applications of solid phase for photoaffinity labeling

研究了生物分子与含生物素衍生物的光反应性三氟甲基苯基二嗪和固定化单体亲和素相互作用的新型功能分析。下面列出了新的发现。(1)光反应性生物素化化合物的合成(2)光反应性生物素化配体在固定化单体亲和标记。(3)利用Friedel-Crafts反应制备生物素化化合物的新方法。

Novel functional analysis of pinteractions between biomolecules and bioactive compounds with photoreactive trifluoromethylphenyldiazirine containing biotin derivative and immobilized monomeric avidin were examined. New findings are listed below.(1) Synthesis of photoreactive biotinylated compounds(2) Photoaffinity labeling with photoreactive biotinylated ligands on immobilized monomeric avidin.(3) Novel synthesis of biotinylated compounds, which was prepared by Friedel-Crafts reactions.