Immobilized Cell Production of Secondary Metabolites

次生代谢物的固定化细胞生产

• 批准号: 8712083
• 负责人: John Gainer
• 金额: $ 21.53万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-15 至 1992-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8712083&HistoricalAwards=false
• 关键词: Immobilized; Cell; Production; Secondary; Metabolites

Narrative: Many of the useful products formed by microorganisms are known as secondary metabolites since they are not required for the growth or primary metabolism of the microbes. Antibiotics such as penicillin and cephalosporin C are examples of secondary metabolites currently produced on a large scale. Products from genetically altered organisms also resemble, for the most part, secondary metabolites. Current production methods involve making the product in individual batches. It would be more efficient to do this in a continuous manner if good processing and separation steps could be developed. The use of immobilized microorganisms (i.e., attaching the organisms to solid supports) presents an attractive approach to continuous processing, and that is the focus of the proposed research. This research will investigate the regulation of secondary metabolite production by immobilized cells through controlling the nutrient levels. Also, it is intended to study the effect of immobilization on the stability of genetically altered microorganisms, as well as the effect on altering the metabolism of attached cells. The results of the research should be extremely useful in developing new continuous production processes for secondary metabolites.

叙述：由微生物形成的许多有用的产品是 它们被称为次级代谢物，因为它们不是代谢所必需的。 微生物的生长或初级代谢。 抗生素如 青霉素和头孢菌素C是次级代谢物的例子 目前大规模生产。 产品从基因 在大多数情况下，改变的生物体也类似于次级生物体。 代谢物。 目前的生产方法涉及单独制造产品 分批 这样做会更有效率， 如果能够开发出良好的加工和分离步骤，则可以采用这种方式。 固定化微生物（即，将这些生物 固体支持物）提出了一种有吸引力的方法， 处理，这是拟议研究的重点。 这 研究将调查次级代谢物的调节 通过控制营养物的固定化细胞生产 程度. 此外，还打算研究固定化的效果 转基因微生物的稳定性，以及 改变附着细胞的新陈代谢的效果。 的 研究结果对开发新的 次级代谢产物的连续生产工艺。