Development of Production Process for Anti-Cancer Drug Taxol with Immobilized Plant Cell Culture.

研究了日本红豆杉游离细胞和固定化细胞生产紫杉醇的特性。由于悬浮培养物中活细胞的分泌，在培养基中观察到大量的紫杉醇。因此，使用具有膜细胞分离器或固定化细胞的连续生产系统，与分批操作相比，紫杉醇生产率提高了十倍。以0. 3 mg-Taxol/g-DCW/d的比生产速率连续生产紫杉醇40天。提出了一种利用红豆杉细胞生产紫杉醇的新工艺。

The production characteristics for Taxol (paclitaxl) using free or immobilized cells of the Japanese yew tree, Taxus cuspidata, were investigated. A considerable amount of Taxol was observed in the medium due to secretion by living cells in the suspension culture. Therefore, using a continuous production system with a membrane cell separator or immobilized cells, Taxol productivity was increased by a factor of ten compared to the batch operation. The continuous Taxol production using immobilized cells was successfully performed at about 0.3mg-Taxol/g-DCW/d of specific production rate for up to 40 days. A novel Taxol production process using cultured cells of the yew tree is proposed.