US-UK Collaborative Research: Oxygen Consumption of Cells and Tissues

美英合作研究：细胞和组织的耗氧量

• 批准号: 8922468
• 负责人: John Gainer
• 金额: $ 0.97万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8922468&HistoricalAwards=false
• 关键词: US; UK; Collaborative; Research; Oxygen

This award will support collaborative research between Dr. John Gainer, University of Virginia and Dr. P.A. Arundel, ICI Pharmaceuticals, Macclesfield, Cheshire, England. The objective of the proposed research is an investigation of oxygen consumption in cells and tissues. The manufacture of products from living cells, whether microbial or animal, depends strongly on a thorough knowledge of the metabolism of such cells. Dr. Gainer's current research at the University of Virginia is concerned primarily with factors affecting the production of secondary metabolites from immobilized microbial cells. Work is also ongoing to define the metabolic needs of free and immobilized animal cells, in particular with respect to the oxygen consumption. It has been found that the oxygen consumption rate per cell is quite similar for both animal and microbial cells, and is dependent on the density of the cells--decreasing as the density increases. The next step is to extend these results to very high cell densities. This can be done, in the case of animal cells, by studying the oxygen consumption of tissues. ICI, at their Pharmaceuticals Research Center near Manchester , England, has an active, interdisciplinary group involved in basic research concerning oxygen consumption and metabolic needs of tissues. During a four month research stay in England, Dr. Gainer proposes to carry out joint studies at the ICI Research Center, which will involve both a study of the factors affecting oxygen consumption in tissues and a comparison of those results to previous data for individual cells. Dr. Gainer has had considerable experience in the mass transfer of oxygen to cells and living systems. The Metabolic Dysfunction Team at ICI has excellent facilities, and has approached the problem of oxygen consumption and metabolism from a more reaction-controlled mechanism. The combination of the two viewpoints, along with the facilities necessary for obtaining data, should complement each other well and allow for a more complete description of the entire oxygen consumption process.

该奖项将支持弗吉尼亚大学 John Gainer 博士和 P.A. 博士之间的合作研究。 Arundel，ICI 制药公司，英国柴郡麦克尔斯菲尔德。 拟议研究的目的是调查细胞和组织的耗氧量。 从活细胞（无论是微生物细胞还是动物细胞）生产产品在很大程度上取决于对此类细胞新陈代谢的透彻了解。 盖纳博士目前在弗吉尼亚大学的研究主要涉及影响固定微生物细胞产生次级代谢产物的因素。 确定游离和固定动物细胞的代谢需求的工作也在进行中，特别是在耗氧量方面。 人们发现，动物细胞和微生物细胞的每个细胞的耗氧率非常相似，并且取决于细胞的密度——随着密度的增加而降低。 下一步是将这些结果扩展到非常高的细胞密度。 对于动物细胞来说，这可以通过研究组织的耗氧量来完成。 ICI 在英国曼彻斯特附近的药物研究中心拥有一个活跃的跨学科小组，致力于有关组织耗氧量和代谢需求的基础研究。 在英国为期四个月的研究期间，Gainer 博士提议在 ICI 研究中心进行联合研究，其中将涉及影响组织耗氧量的因素的研究以及将这些结果与单个细胞的先前数据进行比较。盖纳博士在氧气向细胞和生命系统的传质方面拥有丰富的经验。 ICI 的代谢功能障碍团队拥有优良的设施，并从更受反应控制的机制来解决耗氧和代谢问题。 两种观点的结合以及获取数据所需的设施应该能够很好地相互补充，并能够更完整地描述整个耗氧过程。