Receptor-Mediated Cell Separations

受体介导的细胞分离

• 批准号: 8712784
• 负责人: Douglas Lauffenburger
• 金额: $ 52.21万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-11-01 至 1991-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8712784&HistoricalAwards=false
• 关键词: Receptor; Mediated; Cell; Separations

The exploitation of cell surface receptors for selective cell separation processes holds great promise for a wide range of applications. The fundamental engineering principles and underlying biochemical/biophysical phenomena to be generated by the proposed projects are expected to be generalizable to a very diverse range of potential cell separation processes. In addition to intrinsic receptor/ligand (binding molecule) biochemical interactions, there are a number of biophysical considerations which affect the selectivity and yield of receptor-mediated cell separation processes. The PIs propose a set of complimentary projects aimed at a quantitative understanding of the influence of key parameters on receptor-mediated adhesion of cells to a ligand-coated surface. Specifically, they will investigate the affects of receptor and ligand densities, receptor mobility, receptor/ligand binding affinity, temperature, and fluid shear rate on receptor-mediated cell separations. Model systems will include antigen-coated polymeric microspheres and cultured neuroblastome cells, with appropriate antibody and lectin ligand-coated surfaces. This grant application is an excellent example of the type of cross-disciplinary proposal we wish to see in the Engineering Directorate's Biotechnology Program. The Principal Investigators are considered well qualified to carry out the proposed research and I recommend funding of this proposal for three years in accordance with the revised budget.

细胞表面受体的开发为选择性细胞分离过程提供了广阔的应用前景。预计拟议项目将产生的基本工程原理和潜在的生化/生物物理现象可推广到各种潜在的细胞分离过程。除了固有的受体/配体(结合分子)生化相互作用外，还有许多生物物理因素影响受体介导的细胞分离过程的选择性和产率。PIs提出了一套补充项目，旨在定量了解关键参数对细胞与配体涂层表面受体介导的黏附的影响。具体地说，他们将调查受体和配体密度、受体迁移率、受体/配体结合亲和力、温度和流体剪切率对受体介导的细胞分离的影响。模型系统将包括抗原涂层的聚合物微球和培养的神经母细胞，表面有适当的抗体和凝集素配体涂层。这项拨款申请是我们希望在工程局的生物技术计划中看到的跨学科建议的一个很好的例子。首席调查员被认为完全有资格进行拟议的研究，我建议根据订正预算为这项提议提供三年的经费。