"Quantitative Studies of Binding and Trafficking of Endogenous and Genetically-Altered Epidermal Growth Factor Receptors in Cultured Fibroblasts."

“培养的成纤维细胞中内源性和基因改变的表皮生长因子受体的结合和运输的定量研究。”

• 批准号: 8917010
• 负责人: Douglas Lauffenburger
• 金额: $ 3万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1991-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8917010&HistoricalAwards=false
• 关键词: Quantitative; Studies; Binding; Trafficking; Endogenous

Significant progress has been made during the past decade on identifying the molecular components of the regulation of mammalian cell proliferation by peptide growth factors and their cell surface receptors. Since this regulation underlies many current problems in the reliable and reproducible design of cell culture-based technologies, a translation of this progress into useful quantitative engineering terms is crucial. Preliminary studies are proposed to test the hypothesis that the mitogenic responsiveness of fibroblast cell lines to epidermal growth factor (EGF) is related to the trafficking properties of the EGF receptor. A variety of kinetic EGF binding and dissociation studies will be performed in order to estimate values for various trafficking constants in a mathematical model. These studies intend to determine whether any changes in trafficking parameters are correlated to proliferation responses. If this is so, a substantial research program along these lines will be motivated.

在过去的十年中，在确定肽生长因子及其细胞表面受体调节哺乳动物细胞增殖的分子成分方面已经取得了重大进展。 由于这一规定是当前基于细胞培养技术的可靠和可重复设计中的许多问题的基础，因此将这一进展转化为有用的定量工程术语至关重要。 初步研究旨在检验成纤维细胞系对表皮生长因子 (EGF) 的有丝分裂反应性与 EGF 受体的运输特性相关的假设。 将进行各种动力学 EGF 结合和解离研究，以估计数学模型中各种运输常数的值。 这些研究旨在确定贩运参数的任何变化是否与扩散反应相关。 如果是这样，将会激发沿着这些思路进行的实质性研究计划。