U.S.-Chile Cooperative Research in Isolation of Iron BindingProtein of Reticulocyte Coated Vesicles

该奖项支持路易斯安那州立大学的乔纳森·格拉斯和智利大学的马尔科·图里奥·努涅斯在分离网织红细胞包裹的囊泡中铁结合蛋白的合作研究。这两位科学家进行了长期而富有成效的合作，此外，还与两国的其他研究人员进行了互动。由于格拉斯在铁代谢的细胞生物学方面的专业知识，前两项任务-开发一种检测铁结合蛋白的方法并将其分离-将在他的实验室进行。预计会有快速的进展。努涅斯将参与这一阶段，然后将化验带到智利，在那里将研究电化学梯度对铁在涂层囊泡膜上转移的作用。智利人在跨脂双层传导和跨囊泡膜转运方面拥有路易斯安那州立大学所没有的专业知识。该项目的大部分前期工作已经展开。协作卓有成效，很可能会产生大量数据。这将增加关于网织红细胞中铁跨膜运输的少量知识。由于化验开发将在路易斯安那州立大学进行，因此工作将在智利进行，否则无法进行。这将使智利的大学和那里的学生受益，而路易斯安那州立大学则受益于一位经验丰富的拉丁美洲科学家。

This award supports cooperative research on Isolation of Iron Binding Protein of Reticulocyte Coated Vesicles between Jonathan Glass of Louisiana State University and Marco Tulio Nunez of the University of Chile. These two scientists have had a long and productive collaboration and in addition, interactions with other researchers in both countries. Because of Glass's expertise in cell biology of iron metabolism, the first two tasks, development of an assay to detect an iron binding protein and its isolation will take place in his laboratory. Rapid progress is expected. Nunez will participate in this phase and then take the assay to Chile where the role of electrochemical gradients on translocation of iron across coated vesicle membranes will be studied. The Chileans have expertise in conduction across lipid bilayers and transport across vesicle membranes not available at LSU. Much of the preliminary work on this project has already been carried out. The collaboration has been productive and it is likely that significant data will be generated. This will add to the small amount of knowledge about iron transport across membranes in reticulocytes. Because the assay development will take place at LSU, work will be carried on in Chile which could not otherwise. This will benefit the university in Chile and the students there while LSU benefits from an experienced Latin American scientist.