Biophysical Studies of Vicia Faba Protoplast Ion Channels

植物激素与遗传和环境因素相互作用，调节植物生长、发育和生理的各个方面。尽管进行了大量的努力，但植物激素的作用机制还没有很好地确定。这个项目将研究植物激素对植物质膜离子通道的影响，以努力确定它们的作用模式。膜片电压钳、磁共振和荧光技术将被用来确定基于钙进入或内部钙释放的细胞内控制模型是否能够解释植物激素的不同影响。特别是，来自悬浮细胞培养的蚕豆原生质体和分离的保卫细胞原生质体将被检查以识别离子通道，测量通道的选择性和电压依赖性，并测试电压变化是否可能由产生质子泵的作用、通道激活或泵和通道的组合引起。将测试与刺激相关的保卫细胞体积变化的特定模型。这些研究的结果将大大有助于我们理解植物激素的基本作用机制。

Plant hormones interact with genetic and environmental factors to regulate every aspect of plant growth, development and physiology. Despite intense effort, mechanisms of plant hormone actions are not well defined. This project will examine the effects of plant hormones on plant plasma membrane ion channels in an effort to define their mode of action. Patch voltage clamp, magnetic resonance, and fluorescence techniques will be used to determine if models of intracellular control based on calcium entry or internal calcium release can account for the diverse effects of plant hormones. In particular, suspension cell culture-derived protoplasts and isolated guard cell protoplasts of Vicia faba will be examined to identify ion channels, to measure channel selectivity and voltage dependence, and to test whether voltage changes may arise from the action of electrogenic proton pumps, channel activation, or a combination of pumps and channels. A specific model of stimulus-linked changes in guard cell volume will be tested. The results of these studies should contribute substantially to our understanding of basic mechanisms of action of plant hormones.