Calcium-Dependent Protein Phosphorylation in the Green Alga Mougeotia
绿藻 Mougeotia 中钙依赖性蛋白磷酸化
基本信息
- 批准号:8716224
- 负责人:
- 金额:$ 20.84万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:1988
- 资助国家:美国
- 起止时间:1988-01-01 至 1991-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This research investigates the mechanisms of calcium action in plant cells. Calcium is a very important regulatory molecule in all eukaryotes. It is thought to play a role as an intracellular messenger that couples physiological responses to external stimuli. The calcium binding proteins have been extensively studied in animals. In this project, the potential targets of calcium action will be investigated in plants. The goal of the research is to advance our understanding of how calcium mediates the effects of environmental stumuli on plant cells. The green alga Mougeotia has been selected as the experimental organism because it is an attractive biological model of calcium- dependent regulation in plants. The approach will involve purification and characterization of the molecular targets of calcium in Mougeotia: calcium-stimulated proteins and enzymes. The focus will be on a recently discovered calcium stimulated protein kinase of Mougeotia. The purification and characterization of the protein kinase will be carried out. Particular emphasis will be on the elucidation of the molecular basis of calcium regulation by identifying the calcium-binding effector molecules that regulate the activity. Also, in order to determine the role of this kinase in Mougeotia, in vitro and in vivo phosphorylation assays will be used to identify the substrates of the protein kinase. The effects of phosphorylation on the in vitro activities of these substrates will be assessed. The changes in in vivo substrate phosphorylation levels under conditions that trigger or suppress calcium-dependent responses also will be determined.//
本研究旨在探讨钙在植物细胞中的作用机制。钙是真核生物中非常重要的调节分子。它被认为是细胞内的信使,将生理反应与外界刺激相结合。钙结合蛋白已在动物中得到广泛的研究。在本项目中,将研究植物中钙作用的潜在靶点。这项研究的目的是促进我们对钙如何介导环境对植物细胞的影响的理解。之所以选择绿藻作为实验生物,是因为它是植物钙依赖调节的一个有吸引力的生物学模型。该方法将涉及纯化和表征木苣苔中钙的分子靶标:钙刺激蛋白和酶。重点将是最近发现的一种钙刺激的木犀草蛋白激酶。将进行蛋白激酶的纯化和鉴定。特别强调的是,通过识别钙结合效应分子来调节钙的活性,阐明钙调节的分子基础。此外,为了确定该激酶在木虱中的作用,体外和体内磷酸化试验将用于鉴定蛋白激酶的底物。磷酸化对这些底物体外活性的影响将被评估。在触发或抑制钙依赖反应的条件下,体内底物磷酸化水平的变化也将被确定
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Daniel Roberts其他文献
Determination of the Relative Stability of a Multipolymorph System via a Novel Pure Component Free Energy Calculation
通过新颖的纯组分自由能计算确定多晶型系统的相对稳定性
- DOI:
10.1021/cg3010568 - 发表时间:
2012 - 期刊:
- 影响因子:3.8
- 作者:
Daniel S. Hsieh;Jun Huang;Daniel Roberts;Qi Gao;Alicia T Ng;D. Erdemir;David K. Leahy;Jun Li;Ming Huang;C. Lai - 通讯作者:
C. Lai
36 Human parahippocampal cortex supports spatial binding in visual working memory
36 人类海马旁皮层支持视觉工作记忆中的空间绑定
- DOI:
10.1136/jnnp-2017-bnpa.60 - 发表时间:
2017 - 期刊:
- 影响因子:0
- 作者:
M. Katshu;N. Dundon;Bronson B. Harry;Daniel Roberts;E. Leek;P. Downing;C. Roberts;G. d'Avossa - 通讯作者:
G. d'Avossa
50-V Shock Hazard Threshold
50V 冲击危险阈值
- DOI:
10.1109/tia.2009.2036541 - 发表时间:
2010 - 期刊:
- 影响因子:4.4
- 作者:
Daniel Roberts - 通讯作者:
Daniel Roberts
A comparison of the lowest effective concentration in culture media for detection of chromosomal damage <em>in vitro</em> and in blood or plasma for detection of micronuclei <em>in vivo</em>
- DOI:
10.1016/j.mrgentox.2022.503503 - 发表时间:
2022-07-01 - 期刊:
- 影响因子:
- 作者:
David Kirkland;James Whitwell;Robert Smith;Kiyohiro Hashimoto;Zhiying Ji;Julia Kenny;Naoki Koyama;David P. Lovell;Hans-Jörg Martus;Krista Meurer;Daniel Roberts;Akira Takeiri;Yoshifumi Uno;Bas-Jan van der Leede;Paul White;Andreas Zeller - 通讯作者:
Andreas Zeller
产碱假单胞菌在油菜根圈定殖的动态和分布研究
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
胡小加;刘胜豛;Daniel Roberts - 通讯作者:
Daniel Roberts
Daniel Roberts的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Daniel Roberts', 18)}}的其他基金
REU Site: Research experiences for Deaf Students in Systems Biology and Molecular Signaling- How Cells and Organisms make Decisions
REU 网站:聋哑学生在系统生物学和分子信号传导方面的研究经验 - 细胞和生物体如何做出决策
- 批准号:
1852247 - 财政年份:2019
- 资助金额:
$ 20.84万 - 项目类别:
Standard Grant
Early Irish Fiction: A Definitive Scholarly Edition of Charles Johnstone, The History of Arsaces, Prince of Betlis (1774)
早期爱尔兰小说:查尔斯·约翰斯通《贝特利斯亲王阿尔萨斯的历史》权威学术版 (1774)
- 批准号:
AH/J006106/1 - 财政年份:2012
- 资助金额:
$ 20.84万 - 项目类别:
Fellowship
Nodulin Intrinsic Proteins at the Plant-microbe Symbiotic Interface: Multifunctional Roles in Metabolite and Water Transport in Nitrogen Fixation and Stress Responses
植物-微生物共生界面的结节蛋白内在蛋白:固氮和应激反应中代谢物和水运输的多功能作用
- 批准号:
1121465 - 财政年份:2011
- 资助金额:
$ 20.84万 - 项目类别:
Continuing Grant
The Nodulin 26 Family of Plant Aquaglyceroporins: Transport Properties and Regulation
植物水甘油孔蛋白的 Nodulin 26 家族:运输特性和调节
- 批准号:
0237219 - 财政年份:2003
- 资助金额:
$ 20.84万 - 项目类别:
Continuing Grant
Collaborative Research: Structure and Function of the Nodulin 26 Aquaglyceroporin
合作研究:Nodulin 26 水甘油孔蛋白的结构和功能
- 批准号:
9904978 - 财政年份:1999
- 资助金额:
$ 20.84万 - 项目类别:
Continuing Grant
Laboratory Exercises in the Biochemistry, Immunochemistry, and Molecular Biology of Regulatory Proteins
调节蛋白的生物化学、免疫化学和分子生物学实验练习
- 批准号:
8951666 - 财政年份:1990
- 资助金额:
$ 20.84万 - 项目类别:
Standard Grant
相似国自然基金
当归芍药散基于双向调控Ras/cAMP-dependent PKA自噬通路的“酸甘化阴、辛甘化阳”的药性基础
- 批准号:81973497
- 批准年份:2019
- 资助金额:55.0 万元
- 项目类别:面上项目
蒺藜苜蓿细胞周期蛋白依赖性激酶(cyclin-dependent kinase)对根瘤发育的功能研究
- 批准号:31100871
- 批准年份:2011
- 资助金额:20.0 万元
- 项目类别:青年科学基金项目
Posphoinositide-dependent kinase-1在肿瘤细胞趋化运动和转移中的作用机制
- 批准号:30772529
- 批准年份:2007
- 资助金额:29.0 万元
- 项目类别:面上项目
相似海外基金
Elucidating the novel role of Calcium/calmodulin-dependent protein kinase II (CaMKII) in presynaptic assembly
阐明钙/钙调蛋白依赖性蛋白激酶 II (CaMKII) 在突触前组装中的新作用
- 批准号:
488544 - 财政年份:2023
- 资助金额:
$ 20.84万 - 项目类别:
Operating Grants
Elucidating the novel role of Calcium/calmodulin-dependent protein kinase II (CaMKII) in presynaptic assembly
阐明钙/钙调蛋白依赖性蛋白激酶 II (CaMKII) 在突触前组装中的新作用
- 批准号:
483384 - 财政年份:2023
- 资助金额:
$ 20.84万 - 项目类别:
Operating Grants
Defining the novel functions of calcium/calmodulin-dependent protein kinase II (CaMKII) in synapse assembly and understanding the synaptic consequences of disease-associated CaMKII variants using Caenorhabditis elegans.
定义钙/钙调蛋白依赖性蛋白激酶 II (CaMKII) 在突触组装中的新功能,并使用秀丽隐杆线虫了解与疾病相关的 CaMKII 变异的突触后果。
- 批准号:
476070 - 财政年份:2022
- 资助金额:
$ 20.84万 - 项目类别:
Studentship Programs
Novel treatment for heart failure by inflammasome regulation targeting calcium-calmodulin dependent protein kinase II
通过针对钙钙调蛋白依赖性蛋白激酶 II 的炎症小体调节治疗心力衰竭的新方法
- 批准号:
20K17119 - 财政年份:2020
- 资助金额:
$ 20.84万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Role of calcium/calmodulin-dependent protein kinase II and development of new treatment strategy for coronary spastic angina
钙/钙调蛋白依赖性蛋白激酶II的作用及冠状动脉痉挛性心绞痛新治疗策略的开发
- 批准号:
19K08530 - 财政年份:2019
- 资助金额:
$ 20.84万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Anti-inflammatory approach targeting calcium/calmodulin-dependent protein kinase as a novel therapeutic strategy to the heart failure
靶向钙/钙调蛋白依赖性蛋白激酶的抗炎方法作为心力衰竭的新型治疗策略
- 批准号:
19K23969 - 财政年份:2019
- 资助金额:
$ 20.84万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
The role of calcium/calmodulin-dependent protein kinase II in synaptotoxicity in Alzheimer's disease models.
钙/钙调蛋白依赖性蛋白激酶 II 在阿尔茨海默病模型突触毒性中的作用。
- 批准号:
nhmrc : GNT1165504 - 财政年份:2019
- 资助金额:
$ 20.84万 - 项目类别:
Project Grants
Molecular mechanism for the regulation of activation in calcium/calmodulin-dependent protein kinase II (CaMKII)
钙/钙调蛋白依赖性蛋白激酶 II (CaMKII) 激活调节的分子机制
- 批准号:
9431888 - 财政年份:2017
- 资助金额:
$ 20.84万 - 项目类别:
Involvement of Calcium/ Calmodulin-Dependent Protein Kinase IV in the sympathetic nerve regulation in the heart
钙/钙调蛋白依赖性蛋白激酶 IV 参与心脏交感神经调节
- 批准号:
17K08527 - 财政年份:2017
- 资助金额:
$ 20.84万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigating the Role of Calcium-Dependent Protein Kinase 28 (CPK28)-Associated Proteins in Plant Innate Immunity
研究钙依赖性蛋白激酶 28 (CPK28) 相关蛋白在植物先天免疫中的作用
- 批准号:
515159-2017 - 财政年份:2017
- 资助金额:
$ 20.84万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Master's