Laboratory Exercises in the Biochemistry, Immunochemistry, and Molecular Biology of Regulatory Proteins

该奖项为生物化学系提供资金， 位于诺克斯维尔的田纳西大学 生物化学、化学和生物学专业，有实际操作经验 最先进的生化技术。 重点将 放置在结构分析中使用的方法上， 蛋白质功能的实验，也说明了一些 当前生物学调控原则。 具体方法将 处理蛋白质和多肽纯化;物理，化学 和蛋白质的免疫化学分析;以及分子克隆 和重组DNA技术。 主要文书 为支持该项目而购买的设备将包括：1。高 高效液相色谱分析系统 蛋白质化学实验; 2.记录式温度计 用于蛋白质光谱和酶动力学实验; 3.的板 用于酶联免疫吸附测定的读数器; 4.一种结构性土地免疫化学电泳设备 纯化蛋白和重组DNA的分析 实验; 5.用于DNA和蛋白质的离心转子 实验; 6.用于分析的附加通用设备 上面描述 购买这些仪器将使 让学生们参与一些不常见的实验， 在本科生生物化学实验室完成，并将提供 为今后的研究和教育工作打下坚实的基础。 受赠人正在匹配来自非联邦来源的奖励。

This award provides funds to the department of Biochemistry at the University of Tennessee at Knoxville to give undergraduate majors in Biochemistry, Chemistry and Biology hands-on experience in state-of-the-art biochemical techniques. Emphasis will be placed on methods used in the analysis of the structure and function of proteins in experiments that also illustrate some current principles in regulatory biology. Specific methods will deal with protein and peptide purification; physical, chemical and immunochemical analyses of proteins; and molecular cloning and recombinant DNA techniques. Major instruments to be purchased in support of the project will include: 1. A high performance liquid chromatography and data analysis system for protein chemistry experiments; 2. Recording spectrophotometers for protein spectral and enzyme kinetics experiments; 3. A plate reader for enzyme-linked immunoabsorbent assays; 4. Electrophoresis equipment for structural land immunochemical analyses of purified proteins and for recombinant DNA experiments; 5. Preparative centrifuge rotors for DNA and protein experiments; 6. Additional general equipment for the analyses described above. The acquisition of the instruments will allow the students to participate in experiments that are not commonly done in undergraduate biochemistry laboratories, and will provide a sound foundation for future work in research and education. The grantee is matching the award from non-Federal sources.