Laboratory Exercises in the Biochemistry, Immunochemistry, and Molecular Biology of Regulatory Proteins

该奖项为田纳西大学诺克斯维尔分校生物化学系提供资金，为生物化学、化学和生物学专业的本科生提供最先进的生化技术实践经验。重点将放在实验中用于分析蛋白质结构和功能的方法上，这些方法也说明了调控生物学中的一些当前原理。具体的方法将涉及蛋白质和多肽的纯化；蛋白质的物理、化学和免疫化学分析；以及分子克隆和重组DNA技术。为支持该项目将购买的主要仪器包括：1.用于蛋白质化学实验的高效液相色谱和数据分析系统；2.用于蛋白质光谱和酶动力学实验的记录式分光计；3.用于酶联免疫吸附分析的平板阅读器；4.用于纯化蛋白质的结构免疫化学分析和重组DNA实验的电泳仪；5.用于DNA和蛋白质实验的制备离心机转子；6.用于上述分析的附加通用设备。这些仪器的购置将使学生能够参与到本科生物化学实验室中不常见的实验中，并将为未来的研究和教学工作奠定良好的基础。受赠人匹配来自非联邦来源的奖励。

This award provides funds to the department of Biochemistry at the University of Tennessee at Knoxville to give undergraduate majors in Biochemistry, Chemistry and Biology hands-on experience in state-of-the-art biochemical techniques. Emphasis will be placed on methods used in the analysis of the structure and function of proteins in experiments that also illustrate some current principles in regulatory biology. Specific methods will deal with protein and peptide purification; physical, chemical and immunochemical analyses of proteins; and molecular cloning and recombinant DNA techniques. Major instruments to be purchased in support of the project will include: 1. A high performance liquid chromatography and data analysis system for protein chemistry experiments; 2. Recording spectrophotometers for protein spectral and enzyme kinetics experiments; 3. A plate reader for enzyme-linked immunoabsorbent assays; 4. Electrophoresis equipment for structural land immunochemical analyses of purified proteins and for recombinant DNA experiments; 5. Preparative centrifuge rotors for DNA and protein experiments; 6. Additional general equipment for the analyses described above. The acquisition of the instruments will allow the students to participate in experiments that are not commonly done in undergraduate biochemistry laboratories, and will provide a sound foundation for future work in research and education. The grantee is matching the award from non-Federal sources.