Genome Structure and Replication of a Plant Rhabdovirus

本研究主要有三个目的：1)继续研究植物横纹肌病毒sonchus yellow net virus (SYNV) RNA的结构和组织；2)用SYNV感染烟草原生质体，研究同步复制过程中病毒特异性rna和蛋白的动力学和亚细胞定位；3)从被感染的原生质体中分离出转录酶。目标1的完成将提供植物横纹肌病毒基因组的精确描述，可用于将其结构、功能和进化与相关动物横纹肌病毒进行比较。原生质体感染SYNV（目标2）将允许分析SYNV rna和蛋白质在特定复制阶段的合成动力学，并允许研究它们与宿主多肽和感染期间出现的细胞病变结构的关联。从感染的原生质体中分离出转录酶复合体（目的3），将有助于在体外研究病毒特异性组分的功能以及信使RNA转录和SYNV RNA复制过程中对宿主因子的需求。

This study has three primary objectives: l) to continue studies on the structure and organization of the RNA of sonchus yellow net virus (SYNV), a plant rhabdovirus; 2) to infect tobacco protoplasts with SYNV so that kinetics and subcellular location of the viral specific RNAs and proteins can be investigated during synchronous replication; and 3) to isolate a transcriptase from infected protoplasts. Completion of objective l will provide a precise description of the genome of a plant rhabdovirus that can be used to compare its structure, function, and evolution with related animal rhabdoviruses. Infection of protoplasts with SYNV (Objective 2) will allow analysis of the kinetics of synthesis of SYNV RNAs and proteins at defined stages of replication and will permit investigation of their association with host polypeptides and cytopathic structures that appear during infection. Isolation of a transcriptase complex (Objective 3) from infected protoplasts will facilitate in vitro studies of the functions of viral specific components and the requirement for host factors during transcription of messenger RNAs and replication of SYNV RNA.