Biology of Recognition of Prey by Suctorian Ciliates

吸水纤毛虫识别猎物的生物学

• 批准号: 8718174
• 负责人: Christine Sundermann
• 金额: $ 3.07万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-15 至 1990-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8718174&HistoricalAwards=false
• 关键词: Biology; Recognition; Prey; Suctorian; Ciliates

Suctorian ciliates and their ciliated prey display remarkable cell-cell recognition and interaction phenomena. Prey are recognized by receptors located on the knob of the predator's tentacle. The prey are recognized through components of their cilia. The proposed work will utilize the predator Discophrya collini, and its prey, Tetrahymena. This is a system which is easily manipulated experimentally. The prey protein(s) that are recognized will be identified by monoclonal antibody technology, and characterized biochemically and ultrastructurally. Related prey species will be tested for cross- reactivity. The results will provide the crucial first step in dissecting, at the molecular level, this cell-cell recognition phenomenon. The work constitutes a clever and potentially useful model system for exploring cell-cell recognition phenomena at the molecular level. Insights gained from this project may well turn out to be applicable to cell-cell recognition phenomena in other systems, including higher organisms.

蔗糖纤毛虫及其被纤毛的猎物表现出显著的细胞-细胞识别和相互作用现象。猎物是由捕食者触角上的感受器识别的。猎物是通过其纤毛的组成部分来识别的。这项拟议的工作将利用捕食者Discophrya Collini及其猎物四膜虫。这是一个很容易在实验中操纵的系统。识别出的猎物蛋白(S)将通过单抗技术进行鉴定，并对其进行生化和超微结构表征。相关的猎物物种将接受交叉反应测试。这些结果将为在分子水平上剖析这种细胞-细胞识别现象提供关键的第一步。这项工作构成了一个聪明和潜在有用的模型系统，用于在分子水平上探索细胞-细胞识别现象。从这个项目中获得的见解很可能被证明适用于其他系统中的细胞-细胞识别现象，包括高等生物体。