Predator-Prey Interaction at the Cellular Level in a Protozoan System

原生动物系统中细胞水平的捕食者与被捕食者的相互作用

• 批准号: 9018047
• 负责人: Christine Sundermann
• 金额: $ 13.2万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-15 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018047&HistoricalAwards=false
• 关键词: Predator; Prey; Interaction; Cellular; Level

Investigations of cell recognition in free-living protozoa have, in the past, been limited mainly to mating type recognition in ciliates. One other example that has been partially investigated is the predator-prey relationship between suctorians and their prey. Prey are selected by recognition of surface antigens displayed on the prey's cilia. During proveious work, monoclonal antibodies specific for ciliary antigens of the prey, Tetrahymena, were generated and localized on the prey. The proposed work will utilize these monoclonal antibodies and identify which antigens are responsible for recognition. Inhibition of recognition (and therefore feeding) will be assayed by selectively masking the prey's surface antigens with monoclonal antibodies. In order to characterize the predator's surface receptor that selects prey, monoclonal antibodies directed against the suctorian will be used to block receptors in a set of assays of feeding inhibition that are complementary to the assays mentioned above. Binding of all monoclonal antibodies that are important in recognition/receptor function will be localized at the light and electron microscope levels with labeled secondary antibody. The ciliated larva of the suctorian, which is not recognized as prey, also will be probed with monoclonal antibodies and lectins to determine its similarity to the adult suctorian and to prey. Suctorians are tentacle-bearing protozoa that feed upon other free- living, ciliated protozoa. Some microorganisms that swim against the tentacles of the suctorian are not captured and remain unharmed, while other ciliated protozoa are immediately captured, immobilized, and ingested. A variety of evidence suggests that receptors for prey are located on the tentacle and that these receptors recognize only certain components of the ciliary membrane of the prey. The goals of the proposed research are to understand the specificity of the receptors on the suctorian tentacle involved in recognizing prey vs non-prey, and to identify the proteins of the prey's ciliary membrane that are recognized. The results of this research will contribute to a new understanding of recognition phenomena in protozoa.

在自由生活的原生动物中对细胞识别的研究， 在过去，主要局限于交配型识别， 纤毛虫 另一个被部分调查过的例子是 是吸食者和它们的 猎物 通过识别表面抗原来选择猎物 显示在猎物的纤毛上 在证明工作中，单克隆 对猎物，四膜虫， 产生并定位在猎物身上。 拟议的工作将 利用这些单克隆抗体，并确定哪些抗原 负责识别。 识别抑制（和 因此进食）将通过选择性地掩蔽 猎物的表面抗原与单克隆抗体。 为了 描述了捕食者选择猎物的表面受体， 将使用针对吸盘的单克隆抗体 在一组摄食抑制试验中阻断受体， 是对上述试验的补充。 所有约束力 在识别/受体中重要的单克隆抗体 功能将被定位在光和电子显微镜 标记的二级抗体水平。 有纤毛的幼虫 不被认为是猎物的吸食者也将被探测 与单克隆抗体和凝集素，以确定其相似性 对成年吸盘鱼和猎物的影响。 吸管虫是一种带有触手的原生动物，以其他自由的- 有纤毛的原生动物 一些微生物在水中 吸盘虫的触角没有被捕获， 毫发无伤，而其他纤毛原生动物立即被捕获， 固定住然后被吞下 各种证据表明， 猎物的受体位于触须上， 受体仅识别睫状膜的某些成分 的猎物。 这项研究的目的是了解 吸盘腱上受体的特异性 在识别猎物和非猎物，并确定蛋白质， 猎物的睫状膜被识别。的结果 这项研究将有助于对识别的新理解 原生动物的现象。