A Study of Tissue-, Ontogenetic- and Environmental Specificity of Gibberellin Biosynthesis Mutants in Pisum Sativum

本研究的目的是调查两个 豌豆赤霉素生物合成突变体。 乐与那 突变等位基因阻断赤霉素的生物合成，每一个都在一个特定的水平上。 赤霉素途径中的特定位点。 提出要 定量测定等基因型的组成器官中的赤霉素水平 用毛细管气相色谱法分析了毛细管柱的显影情况， 质谱分析法来 通过监测赤霉素水平， 可以确定组织特异性和个体发育 这些基因的特殊性。 这项研究的意义在于， 确定内源性赤霉素A1的水平， 在调节节间伸展方面是活跃的， 表型。 这项工作产生的结果将奠定 为未来更多的分子研究奠定了基础，他们可能会 导致发现了商业上有用的豌豆，即，矮人 豆荚大小正常的豌豆。

The purpose of the proposed research is to investigate two gibberellin biosynthesis mutants of Pisum sativum. The le and na mutant alleles block the biosynthesis of gibberellins, each at a specific site in the gibberellin pathway. It is proposed to quantify gibberellin levels in constituent organs of the isogenic lines througout development, using capillary gas chromatography- mass spectrometry. By monitoring gibberellin levels it will be possible to determine the tissue-specificity and ontogenetic specificity of these genes. The significance of this research is the potential to determine how the levels of the endogenous gibberellin A1, which is active in regulating internode extension, causes a dwarf pea phenotype. The findings generated by this work will lay the foundation for more molecular studies in the future and they may lead to the discovery of commercially useful peas, i.e., dwarf pea with normal pod size.