Molecular Genetic Analysis of Auxin Transport in Arabidopsis

9604398埃斯特尔拟议工作的长期目标是了解植物的生长和发育，因为它们受到激素生长素的调节。具体地说，这项工作的重点是生长素的运输机制和生长素在植物中的运输调节。大量具有生长素分布和运输特性缺陷或改变的拟南芥突变体是可用的。这一提议是基于突变体在运输过程中不同时间点的顺序，正如对生长素运输抑制剂的反应改变所揭示的那样-几个突变体的特征将超出它们对细胞、组织和器官形态和发育的影响。拟议的实验包括测量生长素结合的生化研究，以及生长素(IAA)运输和分布的测量。还包括对突变体中已知的生长素调节基因的分析。最后，将对几个已突变的转录本和蛋白质的表达进行微观分析，并将克隆更多的基因。这项拟议的工作代表了大量的工作--选择这些目标是为了提供生长素从植物的茎分生组织到根细胞的运动的完整画面。

9604398 Estelle The long-term goal of the proposed work is to understand plant growth and development as they are regulated by the hormone, auxin. Specifically, the work focuses on auxin transport mechanisms and the regulation of auxin movement through the plant. A large number of Arabidopsis mutants with defective or altered auxin distribution and transport characteristics are available. The proposal is based on the order of mutants at different points in the transport process, as revealed by altered responses to inhibitors of auxin transport - several mutants will be characterized beyond the effects they have on cell, tissue, and organ morphology and development. The proposed experiments include biochemical studies to gauge auxin binding, and measurements of auxin (IAA) transport and distribution. Also included is the analysis of known auxin-regulated genes in the mutants. Finally, the microscopic analysis of the expression of several of the transcripts and proteins that have been mutated will be analyzed and additional genes will be cloned. The proposed work represents a large body of work - the goals are selected to provide a complete picture of the movement of auxin from the shoot meristem to root cells in plants.