Single Ion Channels from Teleost Intestinal Epithelium

来自硬骨肠上皮的单离子通道

• 批准号: 8718633
• 负责人: Christopher Loretz
• 金额: $ 18.68万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-15 至 1991-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8718633&HistoricalAwards=false
• 关键词: Single; Ion; Channels; Teleost; Intestinal

The intestinal epithelium of vertebrates serves an important barrier function controlling the movement of ions, nutrients, and water into the animal. The major objective of the proposed project is to determine the mechanisms by which specific ion channels in the intestinal epithelium contribute to the transepithelial chemical and electrical potential profiles which serve as the driving forces for absorption of ions and nutrients. These specific ion channels located in the apical (luminal) and basolateral (blood side) cell membranes, together with ion transporters and the resistance properties of the paracellular pathway between intestinal epithelial cells are the bases of the transepithelial potential profiles. Hormonal and pharmacological agents can alter transport processes through effects directly or indirectly on membrane channels and carriers. Using intracellular microelectrode, patch clamp and reconstituted membrane vesicle techniques, we will examine the voltage- and chemical-dependence of these specific ion channels in the teleost, goby, intestine. These biophysical and physiological investigations will increase understanding of the role of the intestine in fluid and electrolyte balance. The intestine of vertebrate animals serves not only to absorb nutrients from food, but also to regulate salt and water balance. This is particularly clear in seawater-adapted fishes in which there is a tendency for body water to be lost and salts gained from the salty environment. The goby is a small fish that has the physiological ability to adapt to a wide range of salt concentrations in its environment, and therefore is a very useful organism in which to study the ion transport mechanisms underlying the regulation of salt and water balance. Much has been learned about the transport of salts across the whole intestine of this interesting fish, but the detailed pathways and mechanisms of regulation of passage of ions across the membranes of this tissue have not yet been worked out. This study will apply the latest methods of patch clamp electrical recording in combination with biochemical analysis of the intracellular effects of hormones that affect ion channels involved in salt transport. The results of this research will contribute significantly to our understanding of the physiological function of intestinal cells.

脊椎动物的肠上皮是一个重要的屏障 控制离子、营养物质和水进入土壤的运动功能 动物 拟议项目的主要目标是确定 肠上皮细胞中的特定离子通道 有助于跨上皮的化学和电势 - 用作离子吸收的驱动力的分布， 营养素 这些特定的离子通道位于顶端（管腔） 和基底外侧（血液侧）细胞膜，以及离子 转运蛋白和细胞旁途径的阻力特性 肠上皮细胞之间的相互作用是跨上皮细胞的基础， 潜在的侧写 激素和药物可以改变 通过直接或间接作用于膜的转运过程 渠道和运营商。 采用细胞内微电极、膜片钳 和重组膜囊泡技术，我们将研究 这些特定离子通道的电压和化学依赖性 硬骨鱼虾虎鱼肠 这些生物物理和生理 研究将增加对肠道作用的了解 液体和电解质平衡。 脊椎动物的肠道不仅用于吸收 从食物的营养，而且还调节盐和水的平衡。 这 在适应海水的鱼类中， 倾向于身体水分流失和盐获得从咸 环境 虾虎鱼是一种小型鱼类， 能够适应各种盐浓度， 环境，因此是一个非常有用的生物体，在其中研究 盐和水调节的离子传输机制 平衡 关于盐在海洋中的输运，人们已经了解了很多。 这条有趣的鱼的整个肠道，但详细的途径 以及调节离子穿过细胞膜的机制 这种组织还没有被研究出来。 本研究将应用 膜片钳电记录的最新方法， 激素的细胞内效应的生化分析， 影响与盐运输有关的离子通道。 的结果 研究将大大有助于我们了解 肠细胞的生理功能。