RUI: Anaphylatoxin Action in the Rat CNS

过敏毒素C3a和C5a是过敏活性肽 来源于血清补体的第三和第五组分。 当局部给药到大鼠下丘脑时，它们模仿了 神经递质多巴胺和去甲肾上腺素， 修改标准行为测试的能力。 有 两种肽在新鲜脑切片上的特异性结合位点 C3a在突触体组分（神经末梢组分）上 体外）。 C5a已被证明可以刺激两种细胞的释放， 多巴胺和去甲肾上腺素。 初步数据 通过高效液相色谱法得到的结果表明， C3a从纹状体区的突触体释放多巴胺， 前脑 威廉博士的数据是 过敏毒素在中枢神经系统中的活性。 对这些现象的进一步研究可能会揭示它们可能的 大脑的调节功能。 有两个主要目标 威廉博士的研究计划 第一个目标是 描述神经递质的位置和机制 释放大脑中的活动。 神经递质的释放 存在钙阻滞剂的体外物质 通道、GTP结合蛋白和自身受体。第二 目的是将大脑结合位点与特定的 免疫细胞化学和透射法测定神经末梢类型 电镜

Anaphylatoxins C3a and C5a are pharmacologically active peptides derived from the third and fifth components of serum complement. When administered focally to the rat hypothalamus, they mimic the neurotransmitters dopamine and norepinephrine in their respective abilities to modify standard behavioral tests. There are specific binding sites for both peptides on fresh brain slices and for C3a on synaptosome fractions (nerve terminal fractions in vitro). C5a has been shown to stimulate the release of both dopamine and norepinephrine from synaptosomes. Preliminary data obtained by high performance liquid chromatography indicate that C3a releases dopamine from synaptosomes of the striatal region of the forebrain. Dr. William's data are the first report of activities of the anaphylatoxins in the central nervous system. Further study of these phenomena may reveal their possible modulatory function in the brain. There are two main objectives in Dr. William's proposed studies. The first objective is to characterize the locations and mechanisms of neurotransmitter releasing activity in the brain. The release of neurotransmitter substances in vitro in the presence of blockers for calcium channels, GTP binding proteins and autoreceptors. The second objective is to associate the brain binding sites with specific nerve terminal types by immunocytochemistry and transmission electron microscopy.