Instrumentation for an Undergraduate Recombinant DNA Labora-tory.

本科生重组 DNA 实验室仪器。

• 批准号: 8750861
• 负责人: Robert Pohlman
• 金额: $ 5万
• 依托单位: Colgate University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-05-01 至 1989-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8750861&HistoricalAwards=false
• 关键词: Instrumentation; Undergraduate; Recombinant; DNA; Labora

Instrumentation acquired through this project is being used to initiate a two-semester Molecular Biology course in which undergraduates learn recombinant DNA methodology in the laboratory. Major instruments include an ultracentrifuge with two rotors, UV spectrophotometer, power supplies, DNA sequencing apparatus, constant temperature shakers for bacterial growth, a scintillation counter, and two microcomputers with hard disks. The instruments will be used in a series of 10-week laboratory exercises each semester for students in the Junior year. These exercises will acquaint students with the growth of bacteria, bacteriophages and plasmids, the subcloning of phage genes into plasmid DNA, bacterial transformation with recombinant DNA, site-specific mutagenesis, the construction of a genomic library, nucleic acid filter hybridization, and an introduction to DNA base sequence analysis. The course prepares students for active involvement in undergraduate research projects in Molecular Biology in their Senior year. The faculty anticipates that this course will create such enthusiasm that an increased number of students will consider scientific research careers in the fields of molecular and cellular biology.

通过该项目获得的仪器正用于启动 一门两学期的分子生物学课程， 实验室中的DNA重组方法。 主要文书 包括具有两个转子超离心器、紫外分光光度计、电源 供应品，DNA测序仪，恒温振荡器， 细菌生长，闪烁计数器，和两个微型计算机， 硬盘. 这些仪器将在一系列为期10周的 大三学生每学期的实验练习。 这些练习将使学生熟悉细菌的生长， 噬菌体和质粒，将噬菌体基因亚克隆到质粒中 DNA，用重组DNA进行细菌转化，位点特异性 诱变，基因组文库的构建，核酸筛选 杂交，并介绍了DNA碱基序列分析。 该课程为学生积极参与本科教育做好准备。 研究项目在分子生物学在他们的高级年。 的 教师预计，这门课程将创造这样的热情， 越来越多的学生将考虑从事科学研究 在分子和细胞生物学领域。