Instrumentation for the Expansion of the Advanced Recombinant DNA Techniques Laboratory

用于扩展先进重组 DNA 技术实验室的仪器

• 批准号: 9152210
• 负责人: Robert Pohlman
• 金额: $ 2.13万
• 依托单位: Colgate University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9152210&HistoricalAwards=false
• 关键词: Instrumentation; Expansion; Advanced; Recombinant; DNA

The purpose of the above course is to introduce students to state-of-the-art procedures and instrumentation relating to the use and synthesis of oligonucleotides as probes, primers and instruments of mutagenesis. The instrumentation, which includes a DNA synthesizers, high pressure liquid chromatographic systems and DNA tempcyclers, is used in the synthesis of oligonucleotides, the isolation of oligonucleotides, plasmids and PCR-generated DNA fragments using HPLC, and to introduce mutations using oligonucleotides.

上述课程的目的是向学生介绍 最先进的程序和仪器， 寡核苷酸作为探针、引物和 诱变的仪器。 仪器仪表， 包括DNA合成器、高压液体 色谱系统和DNA温度循环仪，用于 寡核苷酸的合成， 寡核苷酸、质粒和PCR产生的DNA片段 使用HPLC，并使用寡核苷酸引入突变。