Instrumentation for the Expansion of the Advanced Recombinant DNA Techniques Laboratory

上述课程的目的是向学生介绍 最先进的程序和仪器， 寡核苷酸作为探针、引物和 诱变的仪器。 仪器仪表， 包括DNA合成器、高压液体 色谱系统和DNA温度循环仪，用于 寡核苷酸的合成， 寡核苷酸、质粒和PCR产生的DNA片段 使用HPLC，并使用寡核苷酸引入突变。

The purpose of the above course is to introduce students to state-of-the-art procedures and instrumentation relating to the use and synthesis of oligonucleotides as probes, primers and instruments of mutagenesis. The instrumentation, which includes a DNA synthesizers, high pressure liquid chromatographic systems and DNA tempcyclers, is used in the synthesis of oligonucleotides, the isolation of oligonucleotides, plasmids and PCR-generated DNA fragments using HPLC, and to introduce mutations using oligonucleotides.