Messenger RNA Processing in Spinach Chloroplasts

菠菜叶绿体中的信使 RNA 处理

• 批准号: 8801451
• 负责人: Margaret Hollingsworth
• 金额: $ 20.15万
• 依托单位: SUNY College at Buffalo
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8801451&HistoricalAwards=false
• 关键词: Messenger; RNA; Processing; Spinach; Chloroplasts

The longterm goal of this research is to discover how gene expression in chloroplasts affects and is itself affected by chloroplast development and biogenesis. This goal will be approached by first studying the processing of multigenic messenger RNAs transcribed within the chloroplast. There are two main objectives to this project. First is to identify precursor and product RNAs from the ATP synthase gene cluster in spinach chloroplasts. In the course of achieving that goal, the primary transcripts from that gene cluster will be identified. The second objective is to observe the levels of these multigenic transcripts and their processing products as the chloroplast develops from its immature beginnings in the seedling stage to the fully developed state that it reaches in the mature leaf. Both of these objectives will be studied with a number of molecular biological techniques, the chief among them being Northern analysis.%%% Chloroplasts are the cell organelles where photosynthesis takes place. Chloroplasts, like the cell nucleus, have genes which direct the synthesis of proteins needed for photosynthesis. mRNA's are the intermediates between genes and proteins. There has been very little research performed on mRNA processing in chloroplasts. Results from this research will serve as the first steps in the elucidation of this complex process.*** //

这项研究的长期目标是发现基因是如何 在叶绿体中的表达影响并且自身受 叶绿体发育和生物发生。 这一目标将 首先研究多基因的加工， 在叶绿体内转录的信使RNA。 该项目有两个主要目标。 一是 鉴定ATP合酶的前体和产物RNA 菠菜叶绿体中的基因簇。 过程中 为了达到这个目的， 集群将被识别。 第二个目标是 观察这些多基因转录本的水平及其 加工产品的叶绿体从其发展 在幼苗阶段不成熟的开始，以充分 它在成熟叶中达到的发育状态。 两 这些目标将与一些分子研究， 生物技术，其中主要是北方 分析。% 叶绿体是光合作用的细胞器 会发生 叶绿体和细胞核一样， 它指导蛋白质的合成 光合作用 mRNA是基因之间的中间体 和蛋白质。 很少有研究 对叶绿体中mRNA加工的影响。 结果从这个 研究将作为阐明的第一步 这个复杂的过程 ** //