Messenger RNA Processing in Chloroplasts

叶绿体中的信使 RNA 加工

• 批准号: 9105726
• 负责人: Margaret Hollingsworth
• 金额: $ 25.17万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105726&HistoricalAwards=false
• 关键词: Messenger; RNA; Processing; Chloroplasts

The purpose of this proposal is to study chloroplast mRNA processing, with an emphasis on mRNA splicing, as a way to enhance our understanding of chloroplast biogenesis and function. Previous results have posed intriguing questions about the processing and splicing of a gene cluster in spinach chloroplasts that encodes four of the nine chloroplast ATP synthase subunits. Preliminary data suggests that chloroplast mRNA splicing may result in multiple branch points within the same intron. One of the three objectives of this proposal is to investigate those preliminary results. The PI has detected splicing activity in spinach chloroplast extracts. Tow objectives of this proposal are dedicated to the stabilization and initial characterization studies on this in vitro splicing activity. It is not known whether splicing is a rate limiting step in the control of gene expression, but it is an indispensable step in the production of several essential gene products. In this proposal, the plan is to study chloroplast splicing both in vivo and in vitro in order to begin to understand this complex process and the factors that regulate it. Chloroplast gene expression is regulated by several factors including light, heat stress, and physiological factors. This work will help to elucidate control points that regulate chloroplast biogenesis and function.//

本研究的目的是研究叶绿体mRNA 加工，重点是mRNA剪接，作为增强 我们对叶绿体生物发生和功能的理解。 先前 研究结果提出了一些有趣的问题， 菠菜叶绿体中的一个基因簇的剪接， 叶绿体ATP合酶9个亚基中的4个。 初步 数据表明，叶绿体mRNA剪接可能导致多个 同一内含子内的分支点。 三大目标之一 这项建议的目的是调查这些初步结果。 的 PI在菠菜叶绿体提取物中检测到剪接活性。 该提案的两个目标是致力于稳定 以及对这种体外剪接的初步表征研究 活动 尚不清楚剪接是否是在细胞内的速率限制步骤。 基因表达的控制，但它是一个不可或缺的步骤， 生产几种必需的基因产物。 在这项提案中， 计划在体内和体外研究叶绿体剪接 为了开始理解这个复杂的过程， 叶绿体基因的表达受到调控 包括光、热应激和生理因素在内的多种因素， 因素 这项工作将有助于阐明控制点， 调节叶绿体的生物发生和功能。