RUI: The Mechanism of mRNP Activation at Fertilization

RUI：受精时 mRNP 激活机制

• 批准号: 8801885
• 负责人: James Grainger
• 金额: $ 25.7万
• 依托单位: Santa Clara University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-15 至 1992-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8801885&HistoricalAwards=false
• 关键词: RUI; Mechanism; mRNP; Activation; Fertilization

The broad objective of this proposal is to elucidate the mechanisms that control the activation of protein synthesis at fertilization in sea urchin eggs. The increase in protein synthesis is mediated by both the activation of stored maternal messenger ribonucleoprotein (mRNPs) and an increase in the activity of the translational machinery. Dr. Grainger proposes to do a detailed study of the factors that control the repression and activation of stored maternal mRNPs. Proteins that are involved in the translational repression of egg mRNPs will be identified by comparing the protein pattern of inactive egg poly(A)+mRNPs and active fertilized poly(A)+mRNPs. In addition, potential candidates for masking factors will be isolated from in vitro activated mRNPs. Antibodies against these mRNP masking proteins will be used to monitor changes in mRNP proteins as egg mRNPs become activated at fertilization. Changes in intracellular calcium and pH trigger ugg activation and the increase in the rate of protein synthesis. Dr. Grainger will alter the calcium and pH levels of unfertilized eggs and measure the effect of these changes on the translational activity of egg mRNPs. Using antibodies against the mRNP masking factors, he will determine which proteins are modified or released by these treatments. %%% The unfertilized egg is a metabolically quiescent, non- dividing cell. Fertilization results in the activation of a series of pathways, including those leading to an increase in the rate of protein synthesis. Dr. Grainger proposes to study the biochemical processes involved in this activation of protein synthesis upon fertilization using the sea urchin as a model system.

本提案的总体目标是阐明 控制蛋白质合成激活的机制， 海胆卵的受精过程 蛋白质的增加 合成是通过激活储存的母体 信使核糖核蛋白（mRNP）和增加 翻译机器的活动。 格兰杰博士提议 对控制压抑的因素进行详细的研究 和激活储存的母体mRNP。 的蛋白质 参与卵mRNP翻译抑制的蛋白质将被 通过比较不活跃卵子的蛋白质模式 poly（A）+mRNP和活性受精的poly（A）+mRNP。 此外，本发明还提供了一种方法， 掩蔽因子的潜在候选者将从 体外活化的mRNP。 针对这些mRNP掩蔽的抗体 蛋白质将用于监测mRNP蛋白质的变化， mRNP在受精时被激活。 变化 细胞内钙和pH触发ugg激活， 蛋白质合成速率的增加。 格兰杰博士会 改变未受精卵的钙和pH值， 这些变化对egg翻译活性的影响 mRNP。 使用针对mRNP掩蔽因子的抗体， 将决定哪些蛋白质被修饰或释放， 治疗。 %%% 未受精的卵子是一个新陈代谢静止的，非- 分裂细胞。受精导致一种 一系列途径，包括导致增加的途径 蛋白质合成速率。 格兰杰博士建议研究 参与蛋白质活化的生化过程 以海胆为模型的受精合成 系统