Regulation of Translation of Sea Urchin Egg mRNPs

海胆卵 mRNP 翻译调控

• 批准号: 9105142
• 负责人: James Grainger
• 金额: $ 24.4万
• 依托单位: Santa Clara University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105142&HistoricalAwards=false
• 关键词: Regulation; Translation; Sea; Urchin; Egg

The broad objective of this proposal is to elucidate the mechanisms which control the activation of stored maternal mRNAs at fertilization in sea urchin eggs. These RNAs are stored as translationally repressed messenger ribonucleoprotein complexes (mRNPs). A change in intracellular Ca or pH levels or the activation of protein kinase C in some manner alters the structure of the egg mRNPs at fertilization, resulting in their translational activation. The PI proposes to analyze in detail the factors that control the repression and derepression of stored maternal mRNPs. mPNP proteins involved in translation repression will be identified by by comparing the protein patterns of purified inactive and active poly A (+) mRNPs. In addition, mRNP binding proteins will be isolated from in vitro activated egg mRNPs. Antibodies against these mRNP masking factors will be used to monitor changes in mRNP proteins upon mRNP activation at fertilization. Protein kinase C activation or intracellular calcium changes result in the unmasking of egg mRNPs. These changes may activate a cascade of protein phosphorylation reactions. The PI will examine the potential role of phosphorylation in the activation of egg mRNPs both in vivo and in a sea urchin in vitro translation system.

本研究的主要目的是阐明在海胆卵受精过程中控制母体mrna激活的机制。这些rna以翻译抑制信使核糖核蛋白复合物（mRNPs）的形式储存。细胞内Ca或pH水平的变化或蛋白激酶C的激活以某种方式改变受精时卵子mRNPs的结构，导致其翻译激活。PI建议详细分析控制储存的母体mRNPs的抑制和抑制的因素。参与翻译抑制的mPNP蛋白将通过比较纯化的无活性和活性聚A (+) mrnp的蛋白质模式来鉴定。此外，mRNP结合蛋白将从体外激活的卵细胞mRNP中分离出来。针对这些mRNP掩蔽因子的抗体将用于监测受精时mRNP激活时mRNP蛋白的变化。蛋白激酶C的激活或细胞内钙的变化导致卵子mRNPs的揭露。这些变化可能会激活一系列蛋白质磷酸化反应。该项目将在体内和海胆体外翻译系统中研究磷酸化在卵细胞mRNPs激活中的潜在作用。