Wound Modulation of Gene Expression in Plants

植物基因表达的损伤调节

• 批准号: 8802167
• 负责人: Eric Davies
• 金额: $ 13万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8802167&HistoricalAwards=false
• 关键词: Wound; Modulation; Gene; Expression; Plants

The primary objective of this research is to characterize the mRNA population of aged pea epicotyls before and after wounding, as a prerequisite to understanding the molecular basis by which plants respond to this pervasive stress. The hypothesis to be tested suggests that wounding causes disruption of the plasma membrane, thereby permitting a transient influx of calcium. Either directly or indirectly calcium than activates some genes, including genes involved in ethylene biosynthesis. Ethylene turns on different genes, including perhaps genes encoding polysaccaridases which cleave oligosaccharins (elicitors) from cell walls, thereby activating yet another set of genes. Wounding constitutes one of the most pervasive of the stresses to which plants are subjected and is caused by physical factors such as wind and hail, and by biological agents such as large herbivores, insects and fungi. Since wounding by pathogens is such a widespread phenomenon, an understanding of plants' responses to this stress would provide the baseline information common to all injurious pathogens, such that the responses specific to pathogenicity could be more clearly identified. Wounding is also performed, albeit unwittingly, in conventional research, especially in attempts to facilitate uptake of exogenous compounds. Results found here might determine how misleading such protocols are.

本研究的主要目的是描述 老化豌豆上胚轴在损伤前后的mRNA群体， 这是理解分子基础的前提， 植物对这种普遍的压力作出反应。 假设是 测试表明，受伤会导致血浆的破坏， 膜，从而允许钙的瞬时流入。 直接或间接地激活某些基因， 包括参与乙烯生物合成的基因。 乙烯 会开启不同的基因， 多糖酶，其将寡糖素（激发子）从 细胞壁，从而激活另一组基因。 创伤是最普遍的压力之一， 植物受到哪些影响以及由哪些物理因素引起，例如 如风和冰雹，以及生物制剂，如大 食草动物昆虫和真菌 由于病原体的伤害是 如此普遍的现象， 对这种压力的反应将提供基线信息， 常见于所有有害的病原体， 可以更清楚地确定致病性的特异性。 虽然是无意的，但也可以在传统的 研究，特别是在试图促进吸收 外源化合物 这里发现的结果可能会决定如何 这类协议是误导性的。