Do Cytoskeleton-bound Polysomes Exist in Plants?

植物中是否存在细胞骨架结合的多聚核糖体？

• 批准号: 9007729
• 负责人: Eric Davies
• 金额: $ 1万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9007729&HistoricalAwards=false
• 关键词: Do; Cytoskeleton; bound; Polysomes; Exist

The broad goals of Dr. Davies' research program are to understand the plant response to wounding all the way from the initial chemical (or electrophysiological) signal produced by the wound, through intracellular regulatory mechanisms, to alterations in the expression of specific genes responsible for the plant's adaptive response. He has observed that within a few minutes after a wound, a number of specific messenger RNAs can be detected, and there is an increase in polysome formation. Unexpectedly, however, even though new messages can be detected within a few minutes of the wound, overall protein synthesis drops by 75%. Dr. Davies has formulated several alternative hypotheses as to mechanisms that might account for this. One of the hypotheses is that there is a regulated translocation of polysomes from one subcellular site at which they are active, to another site where they are inactive. Consistent with this possibility is his observation of a shift in the way polysomes from wounded and unwounded plants sediment. After wounding, a large fraction of polysomes pellet with larger cellular structures. The short-term goals of this project are to identify and localize the proteins that are encoded by the wound- responsive genes and the polysomes that appear after wounding. In particular he will attempt to determine whether the shift in sedimentation properties of the polysomes is caused by association with the cytoskeleton. The question of whether polysome association with the cytoskelton is a biologically significant phenomenon has been controversial for a long time. The results of this project will make a two-fold contribution. First, they may resolve the long- standing controversy over whether association of polysomes with the cytoskeleton in plant cells occurs under physiological conditions (but this is high risk). Second, the results should establish the location of polysomes involved in translation of wound inducible gene products and so enhance knowledge of the translation stage of the wound response.

戴维斯博士研究项目的主要目标是 了解植物对伤害的反应， 产生的初始化学（或电生理）信号 通过细胞内调节机制， 特定基因表达的改变， 植物的适应性反应。 他说，在几个 在创伤后几分钟，一些特定的信使RNA可以被 检测到，并且多核糖体形成增加。 然而，出乎意料的是，即使可以检测到新消息， 在受伤后的几分钟内， 下降了75%。 戴维斯博士提出了几种替代方案 关于可能解释这一点的机制的假设。 之一 假设是，有一个调节易位 多聚核糖体从它们活跃的一个亚细胞位点， 另一个他们不活跃的地方。 符合本 可能是他观察到多聚核糖体 从受伤和未受伤的植物沉积物。 受伤后，A 大部分的多聚核糖体颗粒具有较大的细胞 结构. 该项目的短期目标是确定 并定位由伤口编码的蛋白质 反应基因和受伤后出现的多核糖体。 特别是，他将试图 确定是否在沉降特性的变化， 多聚核糖体是由与细胞骨架的结合引起的。 关于多聚体是否与 细胞分裂素是一种重要的生物学现象， 争议了很久。 该项目的成果将 做出双重贡献。 首先，他们可以解决长期的- 关于多聚核糖体是否与 植物细胞中的细胞骨架在生理条件下发生， 条件（但这是高风险）。 第二，结果应该 确定参与翻译的多聚核糖体的位置， 创伤诱导基因产物，从而提高了对创伤诱导基因产物的认识。 创伤反应的转化阶段。