Lipid Metabolism and Photosynthesis in Fatty Acid Mutants ofArabidopsis

拟南芥脂肪酸突变体的脂质代谢和光合作用

• 批准号: 8803855
• 负责人: John Browse
• 金额: --
• 依托单位: Washington State University
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8803855&HistoricalAwards=false
• 关键词: Lipid; Metabolism; Photosynthesis; Fatty; Acid

SPECIFIC AIMS The overall goal of this project is to understand the role of membrane lipids in the organization and functioning of plant cells and organelles. This project has the following specific aims: 1. To carry out the lipid analyses and labelling studies required to characterize arabidopsis mutants already selected. 2. To investigate the photosynthetic characteristics of those mutants in which the fatty acid compositions of chloroplast lipids are affected. 3. To construct double mutants with more extensive changes in chloroplast fatty acid composition and to study photosynthesis in these also. 4. To screen independent M2 populations for further mutants and alleles of existing mutants. Results so far obtained indicate that these mutants can provide considerable information on the enzymology of desaturation and on the control of cellular lipid metabolism. More importantly the mutants provide a means of directly addressing the question of how the fatty acid composition of thylakoid membranes affects chloroplast structure and function.%%% Increased knowledge in these areas is an essential prerequisite to intelligently manipulating membrane lipid composition by recombinant DNA techniques. For example, because of the possible importance of membrane lipid composition in the temperature responses to plants, a detailed understanding of fatty acid desaturation and the genes which control this process may make it possible to genetically modify economically important crop species to better suit particular environmental conditions.***

具体目标 该项目的总体目标是了解膜脂在植物细胞和细胞器的组织和功能中的作用。该项目有以下具体目标： 1. 进行脂质分析和标记研究，以表征已选择的拟南芥突变体。 2. 研究叶绿体脂质脂肪酸组成受影响的突变体的光合特性。 3.构建叶绿体脂肪酸组成发生更广泛变化的双突变体并研究其光合作用。 4.筛选独立M2群体的进一步突变体和现有突变体的等位基因。迄今为止获得的结果表明，这些突变体可以提供有关去饱和酶学和细胞脂质代谢控制的大量信息。更重要的是，突变体提供了一种直接解决类囊体膜的脂肪酸组成如何影响叶绿体结构和功能的问题的方法。%%%增加这些领域的知识是通过重组DNA技术智能操纵膜脂组成的必要先决条件。例如，由于膜脂成分在植物温度响应中可能很重要，对脂肪酸去饱和和控制该过程的基因的详细了解可能使得对经济上重要的作物物种进行基因改造以更好地适应特定的环境条件成为可能。***