Molecular Genetics and Biochemistry of a Streptomyces Extracellular Esterase

链霉菌胞外酯酶的分子遗传学和生物化学

• 批准号: 8804638
• 负责人: Janet Schottel
• 金额: $ 19.37万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8804638&HistoricalAwards=false
• 关键词: Molecular; Genetics; Biochemistry; Streptomyces; Extracellular

Streptomyces scabies, a gram positive soil bacterium, is the causative organism for scab disease on a variety of underground vegetables. The pathogen produces an extracellular esterase which may be involved in the breakdown of suberin, a waxy polyester compound that covers the surface of the tuber and thus protect the plant against moisture and possibly pathogen invasion. This esterase has been purified and characterized. Its production is induced by zinc which was found to be a component of suberin. The goal of the research is to elucidate the mechanism by which zinc effects this induction. The esterase gene will be cloned and its sequence determined. Transcription of the gene, the stability and translation of the esterase mRNA, and processing/secretion of the esterase will be tested for zinc dependence. Secretion of this esterase by the pathogen will also be studied. This research will provide important basic information of the mechanisms of gene expression and protein secretion in Streptomyces scabies, an agriculturally important pathogen. The results will enhance our understanding of the mechanisms of gene regulation and the molecular biology of host-pathogen interactions. They may also be applied to the protection of economic crops against infection by pathogenic organisms.

疥疮链霉菌是一种革兰氏阳性土壤细菌， 引起各种疮痂病的病原体 地下蔬菜 病原体产生一种细胞外 酯酶可能参与木栓质的分解， 覆盖块茎表面的蜡质聚酯化合物 从而保护植物免受水分的侵害 病原体入侵 这种酯酶已被纯化， 表征了 它的产生是由锌诱导的， 发现是木栓质的一种成分。 研究的目标 是为了阐明锌的作用机制， 诱导 酯酶基因将被克隆并测序 测定 基因的转录、稳定性和 酯酶mRNA的翻译，以及 将测试酯酶的锌依赖性。 分泌 还将研究病原体的这种酯酶。 这项研究将提供重要的基础信息， 基因表达和蛋白分泌的机制， 疥疮链霉菌，一种重要的农业病原菌。 这些结果将加深我们对 基因调控与宿主-病原体分子生物学 交互. 它们也可适用于保护 经济作物抗病原体感染。