Regulation of Plant Gene Expression in Response to Infectionby Streptomyces Scabies

植物基因表达对疥疮链霉菌感染的响应调控

• 批准号: 9111205
• 负责人: Janet Schottel
• 金额: $ 4.99万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9111205&HistoricalAwards=false
• 关键词: Regulation; Plant; Gene; Expression; Response

Streptomyces scabies is a gram positive soil bacterium that is the causative organism for scab disease on a variety of underground vegetables. The scab lesion that forms at the site of infection is composed of a proliferation of suberinized material by the plant. The mechanism by which the organism elicits the formation of the typical scab lesion on the tuber is not well understood, but may involve the production of compounds such as thaxtomin by the pathogen. The goal of this proposal is to begin to study the regulation of expression of plant genes, in response to the infection process. In order to identify these plant regulated genes, a cDNA library will be prepared from tissue with developing scab lesions. Differential hybridization of the library with cDNAs prepared from non-scab plant tissue versus cDNAs prepared from scab tissue will be used to identify genes expressed specifically during lesion formation. Future work will be focused on studying the regulation of the genes and on the identification of the gene products that are made in response to infection.

疥疮链霉菌是一种革兰氏阳性土壤细菌，是多种地下蔬菜疥疮病的致病微生物。在感染部位形成的痂状病变是由植物的亚蜂化物质增殖组成的。这种生物体引起块茎上典型痂状病变形成的机制尚不清楚，但可能涉及病原体产生诸如thaxtomin之类的化合物。本提案的目标是开始研究植物基因表达的调控，以响应感染过程。为了鉴定这些植物调控基因，将从发展中的结痂组织中制备cDNA文库。将该文库与非结痂植物组织制备的cdna与结痂组织制备的cdna进行差异杂交，将用于鉴定病变形成过程中特异性表达的基因。未来的工作将集中在研究这些基因的调控和鉴定对感染作出反应的基因产物上。