5' Processing of Yeast Cytochrome b pre-mRNA

线粒体生物发生需要协调表达 它们的核和线粒体基因组。 的长期目标 本研究的目的是了解控制的本质， 酿酒酵母中的信号， 线粒体蛋白质的产生， 隔间 核呼吸的特征 缺陷型宠物突变体已经确定了几个基因， 用于处理线粒体前体转录物， 在线粒体核糖体上的所得mRNA的翻译。 一组pet突变体描述了核基因CBP1， 编码一个76，500道尔顿的蛋白质，该蛋白质被输入到 细胞器并与细胞色素B的5'端相互作用 前体RNA。 计划纯化CBP 1蛋白， 体外研究与底物RNA的相互作用。 CBP1 基因产生两个polyA+ mRNA，在3'端相差900 个核苷酸 RNA的丰度是如何以及为什么 将研究葡萄糖阻遏的调节。 编码 序列终止于CBP 1的TATAA盒。 该上游 基因表达为1.1 kb的mRNA，也在 分解代谢物控制 该基因的缺失导致表型 类似于线粒体外膜蛋白的缺失 基因 基因的序列，将被确定。 并 该基因转录影响CBP 1的表达， 定义了编码蛋白在细胞中的功能。 目前，我们只粗略地了解了 亚细胞器的功能完整性 保持和调节以响应变化的环境。 在酵母中， 通过改变它们的生长， 条件 作为三个大的酶复合物的 线粒体内膜由编码 在细胞核染色体和细胞器基因组上， 由于媒体可用性而导致的表达变化必须 协调一致。 是转录因子还是翻译因子 能增强或减少细胞核编码的 呼吸链成分？ 这个问题的答案是 这对于理解线粒体 呼吸

Mitochondrial biogenesis requires the coordinate expression of their nuclear and mitochondrial genomes. The long term goal of this research is to understand the nature of the controlling signals in the Saccharomyces cerevisiae that synchronize the production of mitochondrial proteins encoded in the separate compartments. The characterization of nuclear respiratory deficient pet mutants has identified several genes necessary for processing of mitochondrial precursor transcripts and translation of the resultant mRNAs on mitochondrial ribosomes. One group of pet mutants describes the nuclear gene CBP1, which encodes a 76,500 dalton protein that is imported into the organelle and interacts with the 5' end of the cytochrome b precursor RNA. It is planned to purify the CBP1 protein and study the interaction with substrate RNA in vitro. The CBP1 gene produces two polyA+ mRNAs, differing at the 3' end by 900 nucleotides. How and why the abundance of the RNAs is regulated by glucose repression will be investigated. A coding sequence terminates in the TATAA box for CBP1. This upstream gene is expressed as a 1.1 kb mRNA that is also under catabolite control. Deletions of this gene lead to a phenotype similar to deletion of a mitochondrial outer membrane protein gene. The sequence of the gene, will be determined. Does transcription of this gene affect expression of CBP1 and define how does the encoded protein function in the cell. At the present time we have only a sketchy understanding of how the functional integrity of subcellular organelles is maintained and modulated in response to a changing environment. In yeast the production of mitochondrial respiratory-chain components are significantly altered by changing their growth conditions. As three of the large enzyme complexes of the inner mitochondrine membrane are composed of subunits encoded both on nuclear chromosomes and on the organella genome, the change of expression due to media availability must be coordinated. Is it transcriptional or translational factors that enhance and decrease production of nuclearly encoded respiratory chain components? The answer to this question is of great importance for an understanding of mitochondrial respiration.