Engineering Research Equipment Grant: Advanced Laser Fluorescence for Interfacial Mass Transfer Studies

工程研究设备补助金：用于界面传质研究的先进激光荧光

• 批准号: 8807670
• 负责人: Ronald Neuman
• 金额: $ 4.36万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-15 至 1990-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8807670&HistoricalAwards=false
• 关键词: Engineering; Research; Equipment; Grant; Advanced

In this Research Equipment project, an advanced laser fluorescence apparatus will be developed to determine the surface or translational diffusion coefficients of fluorescent-labeled probes in monomolecular films at air/water and oil/water interfaces. This improved apparatus is based on a simple Moire technique for measuring surface diffusion using fluorescence recovery after photobleaching (FRAP) where the film is bleached in a periodic pattern. This improved FRAP technique will avoid many of the problems associated with the radiotracer technique for measuring surface diffusion. This new experimental technique also will permit one to extend the study surface diffusion to interfacial systems previously inaccessible to study by the radiotracer method. In addition, one will be able to separate between flow and diffusional transport processes occurring in monomolecular films. The periodic pattern increases the spatial resolution relative to the conventional single spot FRAP technique, resulting in more accurate diffusion constant determinations. This apparatus will be used to continue previous work on surface diffusion in monomolecular films, in particular, to determine reliable values of the surface diffusion coefficient and its surface concentration and temperature dependence within various monolayer states in order to elucidate the nature of two- dimensional mass transfer at fluid/fluid interfaces. The laser fluorescence apparatus also will be used in other research projects including monolayer structure, L-B films, bilayers, and solid/liquid interfaces.

在该研究设备项目中，将开发出高级激光荧光设备，以确定在空气/水和油/水接口处的单分子膜中荧光标记探针的表面或翻译扩散系数。 这种改进的设备基于一种简单的Moire技术，用于使用光漂白后（FRAP）荧光回收来测量表面扩散（FRAP），该技术以周期性的方式漂白了膜。 这种改进的FRAP技术将避免与用于测量表面扩散的放射性示踪剂技术相关的许多问题。 这项新的实验技术还将允许人们将研究表面扩散扩展到以前无法通过radiotracer方法进行研究的界面系统。 另外，人们将能够在单分子膜中发生的流动和扩散传输过程之间分离。 相对于常规的单点FRAP技术，周期性模式增加了空间分辨率，从而实现了更准确的扩散常数确定。 该设备将用于继续在单分子膜中进行表面扩散的先前工作，特别是确定各种单层状态内表面扩散系数及其表面浓度和温度依赖性的可靠值，以阐明在流体/流体接口处的两维质量转移的性质。 激光荧光设备还将用于其他研究项目，包括单层结构，L-B膜，双层和固体/液体界面。