Research Initiation: Analysis of Kinetics and Application of Enzymes in Organic Solvents

研究启动：有机溶剂中酶的动力学分析及应用

• 批准号: 8808897
• 负责人: Jonathan Dordick
• 金额: $ 6.96万
• 依托单位: University of Iowa
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8808897&HistoricalAwards=false
• 关键词: Research; Initiation; Analysis; Kinetics; Application

The objectives of this research are to evaluate and directly compare the intrinsic, non-diffusion limited kinetics of enzymatic catalysis in organic media to mechanistically identical reactions in aqueous solutions. This study should extend the practical utilization of enzymes as effective catalysts in organic media by elucidating the variables which control enzymatic activity in novel environments. This should eliminate the continued dependence of enzymatic catalysis in organic media to phenomenological investigations and enable quantitative evaluations of enzymatic activity to be performed. In particular, horseradish peroxidase-catalyzed oxidation of p-anisidine will be used as a model reaction to quantitatively evaluate the effect of organic solvents on enzymic catalytic efficiency. Diffusional limitations will be eliminated by immobilization of the enzyme to solid supports and by vigorous agitation in a monophasic organic solvent. Horseradish peroxidase - catalyzed polymerization of phenols in organic media may represent a viable alternative to conventional phenol-formaldehyde resins. The enzymatic approach offers the attractive alternatives of high reaction selectivity and the absence of formaldehyde. In the long term, peroxidase-produced phenolic polymers may find use both as general movolak or resole resins and for more specific uses such as supports for chromatography, molecular weight standards, semiconductor plastics, polymeric dyes, and developer resins.

本研究的目的是评估和直接比较 酶催化的内在的非扩散限制动力学 在有机介质中的反应与在水溶液中的反应在机械上相同 解决方案 本研究将扩大 酶作为有机介质中的有效催化剂， 在新环境中控制酶活性的变量。 这将消除对酶催化的持续依赖 在有机介质中进行现象学研究， 进行酶活性的定量评价。 在 特别是辣根过氧化物酶催化的对茴香胺的氧化 将被用作模型反应，以定量评估效果 有机溶剂对酶催化效率的影响。 扩散 通过将酶固定化， 固体载体并在单相有机溶剂中剧烈搅拌 溶剂后 辣根过氧化物酶催化的酚类聚合 有机介质可以代表常规介质的可行替代物， 酚醛树脂 酶促方法提供了 具有高反应选择性的有吸引力的替代方案， 甲醛。 从长远来看，过氧化物酶产生的酚 聚合物可用作一般的酚醛清漆或甲阶酚醛树脂 树脂和用于更具体的用途，例如用于 色谱法，分子量标准，半导体 塑料、聚合物染料和显影剂树脂。