Activating Biocatalysts for Nonaqueous and Combinatorial Reactions

活化非水反应和组合反应的生物催化剂

• 批准号: 9811352
• 负责人: Jonathan Dordick
• 金额: $ 26.86万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-15 至 2002-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9811352&HistoricalAwards=false
• 关键词: Activating; Biocatalysts; Nonaqueous; Combinatorial; Reactions

This proposal pursues an older observation that the lyophilization of certain enzyme catalysts in the presence of highly ionic, non-buffering salts, e.g., KCl, can increase the catalytic rate in non-aqueous systems by 3-4 orders of magnitude. This has been demonstrated primarily on subtilisin. The specific aims of the original proposal are: To elucidate the mechanism(s) of enzyme activation in organic solvents with saltsTo extend the concept to a broad range of enzymes and reactions. To utilize the activated enzymes for combinatorial biocatalysis. In the revised and funded proposal, Aim 3 has been dropped and a more detailed examination of the mechanism and scope of the phenomenon has been added.

这一建议追求的是一个较早的观察，即在高离子、非缓冲盐的存在下冻干某些酶催化剂，KCl可以使非水体系中的催化速率提高3-4个数量级。这一点主要在枯草杆菌蛋白酶上得到证实。原提案的具体目标是： 阐明有机溶剂中含盐酶的活化机理，将这一概念扩展到广泛的酶和反应。 利用活化的酶进行组合生物催化。 在经修订和资助的提案中，目标3被删除，并增加了对这一现象的机制和范围的更详细审查。