Analysis of Centromere-Specific Genes in Yeast

这个ROW职业进步奖的长期目标是确定真核生物染色体的着丝点区域如何与纺锤体纤维相关联，以确保适当的染色体分离。最后，研究者将研究着丝点在间期细胞核中组织染色体的作用。这个具体项目将利用遗传和生物化学两种方法。抗体将被制备并用于确定各种突变细胞的蛋白质的细胞位置，并将促进与蛋白质相互作用的成分的分离。这些强有力的方法将允许直接研究酵母中着丝点组装和功能的要求。这种方法与经典的遗传方法相结合，使酵母成为一个很好的真核生物系统模型，用于确定细胞成功分裂的分子机制。

The long-term goal of this ROW Career Advancement Award is to determine how the Kinetochore region of a eucaryotic chromosome associates with a spindle fiber to ensure proper chromosome segregation. Eventually, the investigator will investigate the role the kinetochore plays in organizing the chromosomes in the nucleus during interphase. This specific project will make use of both genetic and biochemical approaches. Antibodies will be prepared and used to determine the cellular location of proteins for various mutant cells and will facilitate the isolation of components with which the proteins interact. These powerful approaches will permit direct investigation of the requirements for kinetochore assembly and function in yeast. This approach, combined with classical genetic approaches, make yeast an excellent model eucaryotic system for determining the molecular mechanisms underlying successful cell division.