Research Initiation Awards: Surface-Induced Conformational Changes in Protein Films

研究启动奖：蛋白质膜表面诱导的构象变化

• 批准号: 8910086
• 负责人: Joseph McGuire
• 金额: $ 5.9万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8910086&HistoricalAwards=false
• 关键词: Research; Initiation; Awards; Surface; Induced

This project will attempt to relate the rate or extent of surface- induced conformational changes in adsorbed proteins to their equilibrium adsorbed mass by in situ ellipsometry. Equilibrium isotherms of alpha-lactalbumin, beta-lactoglobulin, and kappa-casein on germanium will be evaluated. Then, the influence of time on conformational changes experienced by these proteins will be quantified by continuously monitoring the film thickness by ellipsometry. It is now well known that high-molecular weight proteins can be damaged by their interactions with surfaces. The extent of damage seems to be time-dependent. It is hypothesized that this damage occurs as the protein unfolds to bring more of its surface into contact with regions of the surface to which they are attracted. This phenomenon is important to both biomedical technology and downstream processing in biotechnology. If the protein unfolds as it interacts progressively strongly with the surface, it seems reasonable to expect the apparent thickness of a film of adsorbed protein to decrease with time and for the amount of damage to correlate with the film thickness. This project will test that hypothesis and lead to a better understanding of the phenomenon and its dynamics.

本项目将尝试用原位椭偏法将吸附蛋白表面诱导构象变化的速率或程度与其平衡吸附质量联系起来。将评估α -乳白蛋白、β -乳球蛋白和α -酪蛋白在锗上的平衡等温线。然后，通过椭偏法连续监测膜厚度，定量时间对这些蛋白质经历的构象变化的影响。现在大家都知道，高分子量蛋白质可以通过与表面的相互作用而被破坏。损伤的程度似乎是随时间变化的。据推测，当蛋白质展开，使其表面的更多部分与它们被吸引的表面区域接触时，这种损害就会发生。这种现象对生物医学技术和生物技术的下游加工都很重要。如果蛋白质随着与表面的逐渐强烈的相互作用而展开，那么我们似乎有理由期望吸附蛋白质的薄膜的表观厚度随着时间的推移而减少，并且损伤的数量与薄膜厚度相关。这个项目将测试这一假设，并导致对这一现象及其动态的更好理解。