Phenotypic Plasticity in Neural Crest Development

神经嵴发育中的表型可塑性

• 批准号: 8809454
• 负责人: Marianne Bronner
• 金额: $ 24.64万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-10-15 至 1992-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8809454&HistoricalAwards=false
• 关键词: Phenotypic; Plasticity; Neural; Crest; Development

These experiments will probe neurotransmitter plasticity in cholinergic neurons. The longterm goals of this research are to understand the cell lineage decisions involved in aspects of neural crest cell differentiation. The preliminary results have shown that cholinergic neurons derived from the ciliary ganglion can acquire adrenergic properties when transplanted into the trunk somites. This research will extend and further characterize this observation and will examine the phenotypic plasticity of other types of neurons. The experimental paradigm utilizes a new cell marking technique for selectively labeling neurons by retrograde transport of flourescent latex microspheres. After labeling, the neurons will then be microinjected onto neural crest pathways in different regions of the embryo using an injection technique developed in this laboratory. In this way, it is possible to introduce cells into either normal or ectopic sites at a variety of developmental stages.

这些实验将探讨胆碱能神经元中神经递质的可塑性。 这项研究的长期目标是了解神经嵴细胞分化方面涉及的细胞谱系决策。 初步结果表明，来自睫状神经节的胆碱能神经元在移植到躯干体节时可以获得肾上腺素能特性。 这项研究将扩展并进一步表征这一观察结果，并将检查其他类型神经元的表型可塑性。 该实验范例利用一种新的细胞标记技术，通过荧光乳胶微球的逆行运输选择性地标记神经元。 标记后，将使用本实验室开发的注射技术将神经元显微注射到胚胎不同区域的神经嵴通路上。 通过这种方式，可以将细胞引入不同发育阶段的正常或异位位点。