U.S.-Chile Cooperative Research: Neural Crest Induction in Xenopus

美国-智利合作研究：非洲爪蟾的神经嵴诱导

• 批准号: 9722460
• 负责人: Marianne Bronner
• 金额: $ 4万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-15 至 2000-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9722460&HistoricalAwards=false
• 关键词: U.S.; Chile; Cooperative; Research; Neural

9722460 Bronner-Fraser This Americas Program award will support Dr. Marianne Bronner- Fraser, California Institute of Technology, in a research collaboration with Dr. Roberto Mayor, Universidad de Chile. The investigators plan to study the effect of expression of a newly isolated gene, NP-1, on the initial formation of neural crest cells. The new gene, isolated and sequenced from avian embryos by Dr. Bronner in collaboration with two other researchers, has no obvious similarities to other known gene families. However, although the chick system has many advantages for experimental embryology, it is difficult to ectopically express gene products in birds. Therefore, the researchers have initiated studies of the Xenopus system, which represents an ideal system in which to pursue ectopic expression of molecules in early development. To that effect. they plan to isolate the Xenopus homologue of NP-!. Their research will combine molecular biological approaches with experimental embryology, with the long-term goal to characterize and understand the function of the gene in neural crest formation. The collaboration will benefit greatly from the combined expertise and research intereqt of both researchers. The proposed experiments will take place in Dr. Mayor's laboratory, who is an expert on the Xenopus system and has a long standing interest in neural crest development. . ***

9722460 Bronner-Fraser 该美洲计划奖将支持加州理工学院的 Marianne Bronner-Fraser 博士与智利大学的 Roberto Mayor 博士进行研究合作。 研究人员计划研究新分离的基因 NP-1 的表达对神经嵴细胞初始形成的影响。 布朗纳博士与另外两名研究人员合作从鸟类胚胎中分离并测序的新基因与其他已知基因家族没有明显的相似之处。 然而，尽管小鸡系统对于实验胚胎学具有许多优势，但在鸟类中异位表达基因产物却很困难。 因此，研究人员开始了对非洲爪蟾系统的研究，该系统代表了在早期发育中追求分子异位表达的理想系统。 为了达到这个效果。他们计划分离出 NP-! 的非洲爪蟾同源物。 他们的研究将分子生物学方法与实验胚胎学相结合，长期目标是表征和理解该基因在神经嵴形成中的功能。 此次合作将极大地受益于两位研究人员的专业知识和研究兴趣的结合。拟议的实验将在 Mayor 博士的实验室进行，他是非洲爪蟾系统的专家，对神经嵴发育有着长期的兴趣。 。 ***